摘要
目的探讨皮肤恶性黑素瘤中诱导型一氧化氮合酶（iNOS）的表达及其临床意义。方法免疫组化方法检测30例皮肤恶性黑素瘤患者肿瘤组织中iNOS蛋白的表达，逆转录聚合酶链反应（RT-PCR）检测其中6例患者肿瘤组织和肿瘤邻近正常组织中iNOSmRNA的表达。结果iNOS在肿瘤组织中无论蛋白或mRNA阳性表达率皆明显高于邻近正常组织（P〈0．05）。其中iNOS蛋白在无转移的恶性黑素瘤中阳性表达率为69．2％，有转移的恶性黑素瘤中阳性表达率为100％。结论iNOS表达异常在恶性黑素瘤发生、发展与转移过程中可能起重要作用。
Objective To investigate the mRNA and protein expression of inducible nitric oxide synthase （ iNOS ） and its clinical significance in cutaneous malignant melanoma （ MM ）. Methods The iNOS protein expression was detected in tissue sections from 30 MM patients using immunohistochemistry method. Reverse transcription polymerase chain reaction （ RT-PCR ） was used to measure the iNOS mRNA expression in MM tissues and adjacent normal tissues from 6 of the 30 MM patients. Results The positive rates of iNOS protein and mRNA were both significantly higher in tumorous samples than those in normal tissues （ 86.7% vs 20.0%, P = 0.000; 4/6 vs 1/6, P = 0.024 ）. The positive rate of iNOS protein was 69.2% in MM without metastasis, significant lower than that （ 100% ） in MM with metastasis. Conclusion The abnormal expression of iNOS may play an important role in the occurrence, development and metastasis of MM.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2007年第4期227-229,共3页
Chinese Journal of Dermatology
