摘要
为探讨Ｔ淋巴细胞及其释放的细胞因子在哮喘发病中的作用，本研究采用酶联免疫吸附法分别测定３８例急性发作期哮喘病人血清可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）、总ＩｇＥ含量以及外周血单核细胞（ＰＢＭＣ）培养上清液中白细胞介素（ＩＬ）－４水平。结果显示：哮喘病人急性发作期血清ｓＩＬ－２Ｒ（５１２．８２×１０３±２６４．６２×１０３Ｕ／Ｌ）及ＰＢＭＣ培养上清液中ＩＬ－４（１８４２．４２±８２４．６０ｎｇ／Ｌ）明显高于正常对照组（２４２．３２×１０３±１０８．６４×１０３Ｕ／Ｌ和４０６．３２±６２．１６ｎｇ／Ｌ，Ｐ＜０．０１）。ＩＬ－４水平增高与血清总ＩｇＥ呈正相关（ｒ＝０．４９２），与ＦＥＶ１％呈显著负相关（ｒ＝－０．８２１，Ｐ＜０．０５）。其中２３例患者每日静脉滴注氟美松５ｍｇ，用药５天后，其ｓＩＬ－２Ｒ及ＩＬ－４分别降至３３２．６１×１０３±１２４．６２×１０３Ｕ／Ｌ和３１４．１０±７２．０８ｎｇ／Ｌ（Ｐ＜０．０５）。提示Ｔ淋巴细胞激活及其释放的ＩＬ－４在哮喘发病中起重要作用。糖皮质激素对哮喘的疗效，可能是部分通过抑制Ｔ淋巴细胞激活及其释放的ＩＬ－４来完成的。
Several studies have suggested that activated T lymphocytes and its derived cytokines are involved in asthma pathogenesis. To inves tigate the role of activated T lymphocytes and interleukin 4 (IL 4) in asthma pathogenesis, we measured soluble interleukin 2 receptor (sIL 2R) and IgE concentrations in serum and production of IL 4 in situumlated peripheral blood mononuclear cell cultures from asthmatics by means of ELISA. The results showed: (1) sIL 2R (512.82×10 3±264.62×10 3 U/L) and IL 4 (1 842.42±824.60 ng/L) levels in acute severe asthmatics were significantly higher than those of controls (242.32×10 3±108.64×10 3 U/L and 406.32±62.16 ng/L, P <0.01); (2) there was a positive relationship between IL 4 and serum total IgE ( r =0.492); there was a significantly negative relationship between IL 4 and FEV 1% ( r =-0.821, P <0.05); (3) after intravenous administration of dexamethasone (DXM) 5 mg daily for 5 days, sIL 2R and IL 4 decreased respectively to (332.61×10 3±124.62×10 3 U/L and 314.10±72.08 ng/L, P <0.05). These data indicate that activated T lymphocytes and its derived IL 4 play an important role in asthma pathogenesis and that the effect of steroid on the treatment of asthma is partly due to inhibition of activated T lymphocytes and IL 4 release.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1997年第2期102-104,共3页
Chinese Journal of Internal Medicine
