摘要
使用石油醚、乙酸乙酯、无水乙醇和水依次对抗性水稻品种IR36乙醇提取物进行萃取，并测试了4种萃取物对褐飞虱的活性。结果发现乙酸乙酯萃取物处理后3d的活性最强，褐飞虱1～2龄若虫和3～4龄若虫的死亡率分别是26．0％和48．0％。乙酸乙酯萃取物经柱层析分离得到麦黄酮。将麦黄酮定量加入人工饲料中饲养褐飞虱3龄若虫15d。结果表明，褐飞虱3、4龄若虫的排蜜露量随着麦黄酮在饲料中浓度（50～500μg／ml）的增加而减少，褐飞虱3、4龄若虫的死亡率却随着麦黄酮在饲料中浓度的增加而显著提高。当饲料中麦黄酮的浓度为500μg／ml时，褐飞虱3、4龄若虫的死亡率分别为58．21％和31．75％。麦黄酮50～500μg／ml浓度处理，褐飞虱3龄和4龄若虫的发育历期和相对生长量与对照之间没有明显的差异，表明麦黄酮有拒食作用。用500μg／ml的麦黄酮溶液涂抹到对褐飞虱敏感的水稻品种TN1植株上对褐飞虱雌成虫有明显的拒食作用和忌避产卵作用。本项研究结果表明水稻源的麦黄酮在水稻对褐飞虱的抗性中有重要的作用。
Various agronomic traits of crops, particularly in resistance to significantly with crop secondary metabolism and metabolite. We sought, in basis of resistance of rice plants to Nilaparvata pathogen, pest and weed, should correlate instance, to determine the biochemical lugens. The aerial part of resistant rice plant （IR36） was immersed with 85% ethanol in the room temperature and ethanol-soluble extracts were prepared. Different solvents ether, ethyl acetate, absolute ethanol and water were then used to extract the fractions were obtained. These fractions were tested for effects on mortality fractions had significantly impacts on mortality of brown planthopper nymphs 3 ethanol-soluble extracts including petroleum and different solvent of the brown planthopper nymphs. EtOAc d after treatment, with corrected mortality of 26.0% and 48.0% being recorded for 1^st, and 2^nd instar nymphs at 2000mg/L concentration, and 3^rd and 4^th instar nymphs at 3000mg/L concentration, respectively. EtOAc fractions of resistant rice plants （IR36） were separated by column chromatography over silica gel and 5, 7, 4＇ -trihydroxy-3＇ , 5 ＇-dimethoxflavone（tricin）was isolated.Artificial diets containing tricin of different concentrations （ 50 - 500 μg/ml） were prepared to feed on nymphs of the brown planthopper in test-tubes. Twenty 3rd instar nymphs were then introduced into each test-tube for bioassay. After the brown planthopper nymphs fed15 days on diets containing tricin, weight of honeydew of the nymphs reduced as tricin concentrations in the diets increased. The mortality rate of the nymphs increased with increasing tricin concentration. The corrected mortality was 58. 21% for the 3^rd instar nymphs and 31.75% for 4^th instar nymphs though respectively at 500μg/ml concentration. However, even at 500μg/ml concentration the adding weight and development period of 3^rd instar nymphs and 4^th instar nymphs were no significantly different between treatments. Additionally, tricin （500μg/ml） significantly inhibited oviposition and feeding of the brown planthopper females in choice tests when spread on susceptible rice plant （ TN1 ）. Our results suggested that tricin in resistant rice plant played an important role in resistance to brown planthoppers.
出处
《生态学报》
CAS
CSCD
北大核心
2007年第4期1300-1307,共8页
Acta Ecologica Sinica
基金
国家"973"资助项目(G2000016209)
广东省"十五"重大专项资助项目(2004A2042001)~~
关键词
水稻
麦黄酮
褐飞虱
植物抗性
Oryza sativa
tricin
Nilaparvata lugens （StM）
resistence
