摘要
目的:探讨AKT的功能活化状态p-AKT及抗凋亡蛋白Survivin、Bcl-2在非小细胞肺癌(NSCLC)中的表达及相关性。方法:应用免疫组化的方法,检测80例NSCLC组织及35例非肿瘤性肺组织标本中p-AKT、Survivin、Bcl-2的表达,并与临床病理因素进行相关性分析。结果:p-AKT、Survivin、Bcl-2在NSCLC中高表达,阳性率分别为78.8%、61.3%、50.0%,显著高于在非肿瘤性肺组织中阳性表达0、0、11.4%(P<0.05)。p-AKT、Bcl-2在不同年龄、性别、TNM分期、组织分化组以及有无淋巴结转移组均高表达,组间对比差异无统计学意义(P>0.05)。Survivin表达与TNM分期、组织的分化程度有关(P<0.05)。p-AKT阳性表达与Survivin、Bcl-2呈正相关;Survivin与Bcl-2二者也呈正相关。结论:在NSCLC中存在AKT的活化,Survivin、Bcl-2的高表达可能与AKT的活化有关。在NSCLC中可能存在p-AKT对Survivin、Bcl-2的正向调控。
Abstract Objective： To investigate the expression and clinical significance of p-AKT, survivin and Bcl-2 in human non-small cell lung cancer （NSCLC） tissue. Methods： The expressions of p-AKT, survivin and Bcl-2 in 80 cases of NSCLC and 35 cases of no-cancerous lung disease were assessed by immunohistochemistry, and their correlations with cliniopathologi- cal factors were statistically analyzed. Results： The positive rates of p-AKT, survivin and Bcl-2 were 78.8%, 61.3% and 50.0% in NSCLC and 0,0 and 11.4% in no-cancerous lung disease （P〈0.05）. The positive rates of p-AKT and Bcl-2 were showed with a similar high frequency in different age, gender, different histological type, differentiation, TNM stage and groups with or without lymph node metastasis. There were no significant differences among groups （/〉〉0.05）. The expression of survivin was correlated with TNM stages and differentiation of NSCLC （P〈0.05）. In addition, the positive expression of p-AKT had a correlation with the expression of survivin and Bcl-2. The positive correlations were observed between the expressions of sur- vivin and Bcl-2 in NSCLC tissues. Conclusion： AKT activation may present in NSCLC tissues. The high positive rates of survivin and Bcl-2 may correlate to activation of AKT. There may be positive regulations of survivin and Bcl-2 by p-AKT in NSCLC tissues.
出处
《天津医药》
CAS
北大核心
2007年第4期241-243,I0002,共4页
Tianjin Medical Journal
基金
国家自然科学基金资助项目(项目编号:30571552)
河南省教育厅自然科学研究项目(项目编号:2006320049)
