摘要
目的:构建脱细胞猪主动脉瓣膜支架,为体外构建组织工程心脏瓣膜提供适宜的支架材料。方法:新鲜猪主动脉瓣叶,采用“低渗液-0.5%SDS-核酸酶”处理,进行大体、光镜、电镜观察和DNA含量测定。结果:“低渗液-0.5%SDS-核酸酶”可以完全脱去瓣膜细胞,同时较好地保持了胶原纤维和弹力纤维的原有排列和形态。结论:本方法可以成功构建脱细胞猪主动脉瓣膜支架,可进一步用于体外构建组织工程心脏瓣膜的研究。
Objective： To construct an acellular valve scaffold from porcine aortic valves for tissue engineering of heart valve. Methods： A fresh porcine aortic valve leaflet was decellularized by ＂low osmotic pressure of Tris buffer-0.5%SDS-RNase and DNase＂, and assessed by histology, scanning electron microscopy, and the levels of DNA content to confirm the removal of cells. Results： The cells of porcine aortic valve leaflet were removed effectively by ＂low osmotic pressure of rids buffer-0.5%SDS- RNase and Dnase＂ methods. The collagen and elastin were kept intact. Conclusion： An acellular valve scaffold can be obtained successfully through this procedure.
出处
《天津医药》
CAS
北大核心
2007年第4期278-280,I0003,共4页
Tianjin Medical Journal
基金
天津市卫生局科技基金课题(项目编号:01KYGG04)
天津市应用基础研究计划项目(项目编号:06YFJMJC15300)
关键词
细胞外基质
支架
十二烷硫酸钠
组织工程
心脏瓣膜
人工
猪
extracellular matrix
stents
sodium dodecyl sulfate
tissue engineering
heart valve prosthesis
swine
