摘要
应用ＰＣＲ扩增ＨＬＡＤＱＡ１基因第二外显子多态区基因片段，γ３２Ｐ末端标记９个序列特异性寡核苷酸（ＳＳＯ）进行斑点杂交，本文首次对９８例中国人Ⅱ型糖尿病（ＮＩＤＤＭ）与ＨＬＡ的关联进行研究，结果显示ＮＩＤＤＭ全组及常见型亚组０４０１等位基因频率均较正常对照组显著减少，ＰＣ值分别小于０．０１和０．０５，ＲＲ０．２４７，这与ＩＤＤＭ中ＤＱＡ１等位基因频率的变化不同，提示ＮＩＤＤＭ与ＨＬＡ确有关联，但和ＩＤＤＭ发病与ＨＬＡ的关联并不相同。
The association of HLA DQA1gene with type 2 diabetes (NIDDM) was investigated in 132 unrelated chinese subjects(98 NIDDM and 34 normal controls). After amplification with polymorase chain reaction, the fragment of second exon polymorphic region of HLA DQA1gene was hybridized with 9 sequence specific oligonucleotide(SSO) labeled with γ 32 P ATP. The result showed that the frequency of allele 0401 decreased significantly in NIDDM whole group(T group) and A group(NIDDM patients whose age of onset was over 65 yr) (P C<0.01 and 0.05 respectively, RR=0.24). The clinical phenotype of NIDDM A group was consistent with typical NIDDM so that it might be exactly to say that HLA DQA1 gene was associated with NIDDM, and DQA1 0401 might protect individuals from development of NIDDM.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1997年第1期11-14,共4页
Chinese Journal of Endocrinology and Metabolism
基金
卫生部科研基金
