摘要
目的 观察小鼠烧伤后早期类固醇受体辅活化子（SRC）蛋白表达的时相变化，初步探讨烧伤早期糖皮质激素抵抗的分子机制。方法 将30只健康C57／129雄性小鼠随机分为正常对照组与烧伤组，烧伤组给予背部TBSA15％～20％Ⅲ°烧伤。分别于伤后1、6、12、24小时采用Westernblot测定肝、脾组织SRC家族（SRC-1、GRIP-1、NC0A-3）蛋白表达水平的变化。结果 烧伤组肝组织SRC-1蛋白表达水平在烧伤后l小时即显著降低，6小时降至最低，以后逐渐恢复，至24小时与正常对照组相比无显著差异；正常脾组织SRC-1蛋白表达水平较低，测定结果无统计学意义。烧伤组肝组织GRIP-1、NC0A-3蛋白表达水平在烧伤后显著升高，12小时达到高峰，24小时仍显著高于正常对照组；烧伤组脾组织GRIP-1、NC0A-3在烧伤后1小时显著升高，GRIP-1在烧伤后6小时达到高峰，24小时与正常对照相比差异不显著；而NC0A-3在烧伤后12小时达到高峰，24小时显著高于正常对照组。结论 小鼠烧伤后早期SRC-1显著降低，GRIP-1、NC0A-3显著升高，烧伤后糖皮质激素抵抗的发生发展可能与SRC家族成员表达变化有关。
Objective To observe the changes of expression of steroid receptor coactivators （SRC） during the early stage in the burned mice,and the mechanism of glucocorticoid resistance（GCR）. Methods Thirty C57/129 male mice were divided randomly into the control and the bum groups. After clipping of the hair, 15% - 20% thickness Ⅲ°bum injury was produced. At 1,6,12,24h after bum,the levels of hepatic and splenic steroid receptor coactivators（ SRC-1 ,GRIP-1 and NCoA-3 ） were detected by western blot. Results The level of hepatic SRC-1 was markedly down-regulated lh after injury,and reached the lowest point 6h after injury,and gradually returned to the normal level 24h after bum injury. The expression of splenic SRC-1 of the control group was a little lower,which was not statistically significant. The hepatic GRIP-1, NCoA-3 in the burned mice was significantly elevated compared with that of the control group,and peaked 12h after injury. The splenic GRIP-1 ,NCoA-3 was markedly elevated lh after injury,and GRIP-1 was peaked 6h after injury,and gradually returned to the normal level,but NCoA- 3 was peaked 12h after bum,and still obviously higher than that of the control group 24h after bum. Conclusion The SRC-1 was markedly down-regulated,and the GRIP-1 ,NCoA-3 was markedly elevated during the early stage of bum injury,which indicates that the glucocorticoid resistance after bum injury may be associated with the changes of expression of steroid receptor coactivators.
出处
《创伤外科杂志》
2007年第3期264-267,共4页
Journal of Traumatic Surgery
基金
国家重点基础研究发展规划项目("973"项目)(G1999054201)
海外青年学者合作研究基金(30328025)
关键词
烧伤
类固醇受体辅活化子
糖皮质激素抵抗
bum injury
steroid receptor coactivators
glucocorticoid resistance
