摘要
目的：以天麻块茎为材料，研究不同的预处理方法对DNA提取的纯度和浓度的影响。方法：将新鲜天麻块茎切成薄片，分别置于生理盐水，灭菌双蒸水及不同浓度的乙醇溶液中，于4℃存放5～7d，再分别用CTAB法和SDS法提取其总DNA。结果：样品采用浓度为30％～50％的乙醇溶液浸泡后再提取，获得的DNA浓度和纯度都较为理想，而经生理盐水和灭菌双蒸水预处理后提取的样品DNA虽纯度不理想，但浓度较高。结论：采用30％～50％的的乙醇溶液可作为天麻块茎总DNA提取前的预处理液，生理盐水和灭菌双蒸水适用于样品的长期保存。
Objective： Different pretreatment methods affecting purity and concentration of DNA extraction of Rhizoma Gastrodiae tuber were studied. Methods： Tubers of fresh samples were cut into thin pieces and placed in physiologic saline, sterilized ddH2O or ethanol of different concentrations respectively. After them being stored at 4℃ for 5 - 7 days, genomic DNA was extracted with CTAB and SDS methods. Result： Both purity and concentration of DNA extracted with pretreatment of 30%- 50% ethanol were more ideal, while the DNA extracted with pretreatment of physiologic saline or sterilized ddH20 showed higher concentration but lower purity. Conclusion： 30% -50% etnanol pretreatment is better for DNA extraction of Rhizoma Gastrodiae tuber, while physiologic saline and sterilized ddH20 are suitable for long -term sample storing.
出处
《贵阳医学院学报》
CAS
2007年第2期123-125,130,共4页
Journal of Guiyang Medical College
基金
科技部2004BA721A33
贵州省科技黔科合农社字(2001)1125号
黔科合中药专字[2003]51号
关键词
天麻
DNA
乙醇
氯化钠
Gastrodiae elata
DNA
ethanol
sodium chloride
