摘要
研究了8-羟基喹啉与VB12中钴离子的显色反应,实验证明在pH4.0～9.0的NaH2PO4-K2HPO4缓冲溶液中8-羟基喹啉与钴均能生成黄色络合物,在λmax=420nm处钴含量在0～1μg/mL的范围内符合比耳定律。以所拟方法用氯仿为萃取剂可测定VB12药片以及发酵液中的VB12,结果良好。
Color reaction of 8-Hydroxyquinoline with cobalt（Ⅱ） was studied. In NaH2PO4-K2HPO4 buffer solution （pH4.0-9.0）. it can react with Co（Ⅱ） and get a yellow colored complex, and absorption maxima at 420nm, The concentration range of 0-1μg/mL Co（Ⅱ） solution obeyed Beer＇s law, This method can be applied to the determination of VB12 in pill and fermentation solution with a satisfactory result.
出处
《食品科技》
CAS
北大核心
2007年第4期203-205,共3页
Food Science and Technology
