摘要
采用常规化学分析和微生物群落变性梯度凝胶电泳(DGGE)监测技术,探讨投加不同价态铁元素对硫酸盐还原过程的影响以及相应的微生物群落动态响应.结果表明,反应器启动后5d内硫酸盐去除率达到80%,在此过程中群落条带逐渐减少,但与已分离的硫酸盐还原菌(SRB)菌株一致的条带并没出现;Fe3+的投加极显著地改变了原有的高效群落结构,硫酸盐去除率降至20%,氧化还原电位(ORP)有所上升;而Fe2+和Fe0的投加未改变已经形成的顶极群落结构模式,也未显著降低硫酸盐去除率,仅硫化物的浓度变化对Fe2+的投加有短暂的响应.
The influence of different valence state Fe element on sulfate reduction process and the dynamic response of corresponding microbial community were explored by conventional chemical analyses and microbial community denatured gradient gel electrophoresis （DGGE） technique. Only 5d after the starting of reactor, the sulfate-removal rate reached 80%, in this process, the community bands decreased gradually, but the already departed corresponding band of the sulfate-reducing bacteria （SRB） did not appear. The addition of Fe^3＋ altered remarkably the structure of original, high efficient community. The sulfate-removal rate reduced to 20% and oxygen reduction potential （ORP） had a little increase; but the addition of Fe^2＋ and Fe^0 did not alter the structure model of the extreme community. Already formed sulfate-removal rate also did not reduce markedly and only the concentration change of sulfide had short response on the addition of Fe^2＋.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2007年第2期199-203,共5页
China Environmental Science
基金
国家自然科学基金资助项目(50208006)
关键词
硫酸盐还原
铁元素
微生物群落
变性梯度凝胶电泳
sulfate reduction
Fe element
microbial community
denaturing gradient gel electrophoresis （DGGE）
