摘要
目的:纯化蚕蛹油中α-亚麻酸并建立含量测定方法。方法:采用尿素包合法富集α-亚麻酸并用均匀设计法优化筛选其最佳工艺条件,气相色谱内标法测定蚕蛹油中α-亚麻酸的含量。结果:尿素包合后,蚕蛹油中α-亚麻酸的含量由31.0%提高到58.3%;α-亚麻酸浓度在0.125～4.000mg·mL^(-1)范围内线性关系良好(r=0.9999),平均回收率为99.8%。结论:尿素包合法能有效提高蚕蛹油中α-亚麻酸含量;气相色谱内标法用于测定蚕蛹油中α-亚麻酸的含量,方法简便、准确、可靠,可用于α-亚麻酸及其制剂的质量控制。
Objective：Enriching the α-linolenic acid in silkworm pupa oil and establishing a determination method of its content. Methods： Purifying the α- linolenic acid by urea adduct method and using uniform - design to choose the best technology condition; employing a gas chromatography （ GC ） - internal reference method to determine the content of α- linolenic acid in silkworm pupa oil. Results： After urea adducting, the content of α- linolenic acid in silkworm pupa increases from 31.0% to 58.3% ;the linear correlation of α- linolenic acid＇s concentration from 0. 125 to 4. 000 mg· mL^-1 is fine（r =0. 9999） ;the average recovery is 99. 8%. Conclusion：As a result urea adduct method can enhance the content of α- linolenic acid in silkworm pupa oil effectively; GC - internal reference method using to determine the content of α- linolenic acid in silkworm pupa oil is simple, exact and reliable. So it can be used to control the quality of α- linolenic acid and its pharmaceutical preparation.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第4期535-538,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
蚕蛹油
Α-亚麻酸
尿素包合法
气相色谱法
silkworm pupa oil
α- linolenic acid
urea adduct method
gas chromatography
