摘要
目的:通过观察丹参(SM)对急性肺损伤(ALI)大鼠肺水通道蛋白-1(AQP-1)的影响,探讨其保护作用。方法:SD大鼠48只,随机分为正常组、丹参注射液预保护组、丹参注射液7 d治疗组、ALI 7 d模型组、丹参注射液8hr治疗组、ALI 8 hr模型组,每组各8只。分别于7 d和8 hr后电镜HE染色观察各组大鼠肺组织病理改变,肺湿/干重比(W/D)观察肺脏水通透性的变化,免疫组化法及肺组织匀浆后Western blotting法检测AQP-1表达变化。结果:模型组均出现肺损伤症状。SM7 d治疗组的肺水肿病理改变与ALI 7 d模型组比较明显减轻,但两组AQP-1表达均与正常组无明显差异(P<0.05)。SM8 hr治疗组和SM预保护组与ALI 8 hr模型组比较肺水肿病理改变明显减轻,血流变明显改善(P<0.05),其中SM预保护组AQP-1表达上调明显,有显著差异(P<0.01)。结论:ALI时SM能增加肺组织毛细血管上AQP-1的表达,从而减轻其减少引起的肺水肿,在一定程度上缓解ALI病变的发生与发展。
Objective ： To investigate the effect of SM on aquaporin-1 （ AQP1 ） expression in rats with acute lung injury （ ALI）, and study protection against ALI Methods ： 48 SD rats were randomly divided into normal group, ALI 7-day group, ALI 8-hour group, SM prevention group, SM 7-day treatment group, and SM 8-hour treatment group. At 8 hours and 7 days after treatment, lung wetto-dry weight ratio, lung pathology, electron microscope, AQP1 immunohistochemistry, and AQP1 Western blot were performed. Results： The model group rats all appeared ALI symptom. Compared with the 7-day ALI group, the degree of lung edema statement alleviated in SM 7-day treatment group. But the expression of AQP-1 was not significant between the two groups （P 〈 0. 05 ）. Compared with the 8- hour ALI group, the degree of lung edema statement alleviated in SM 8-hour treatment group and SM prevention group. The expression of AQP-1 was significantly expression in the group of SM prevention group （ P 〈 0. 01 ）. Conclusion ： SM may improve the body fluid metabolism and alleviate the degree of the lung edema by regulating the expression of AQP-1. The results suggest SM can regulate the activity of AQP-1 to improve the water metabolism of ALI.
出处
《中药材》
CAS
CSCD
北大核心
2007年第4期442-444,共3页
Journal of Chinese Medicinal Materials
基金
西京医院创新基金(XJCX05M29)
关键词
丹参
急性肺损伤
水通道蛋白
保护作用
Salvia mihiorrhiza
Acute Lung Injury
Aquaporin-1
Protection
