摘要
目的探讨人新基因溶酶体相关4次跨膜蛋白质B(lysosome-associated protein transmembrane 4 beta,LAPTM4B)多态性与淋巴瘤易感性的关系。方法以病例-对照研究的方法,基于特异性引物的PCR对350例正常人和166例胃癌患者进行LAPTM4B基因分型,用χ2检验检测淋巴瘤组和对照组基因型频率和其他参数的分布。结果淋巴瘤组中LAPTM4B基因的*2等位基因的频率为34.94%,较对照组(24.14%)显著增高(P<0.001)。基因型分布在淋巴瘤组与对照组间差异有统计学意义。*1/2和*2/2基因携带者患淋巴瘤的危险性分别是*1/1的1.475倍(95%CI:0.994-2.189)与3.532倍(95%CI:1.802-6.923)。*2等位基因携带者患淋巴瘤的危险性是*1等位基因的1.710倍(95%CI:1.287-2.271)。LAPTM4B基因型分布与患者年龄、性别、病理类型、临床分期、HBV感染、乳酸脱氢酶(LDH)、β2-微球蛋白(β2-MG)以及有无全身症状等参数无明显关系。结论LAPTM4B基因多态性与淋巴瘤易感性相关,*2等位基因可能是淋巴瘤发生的危险因素。
Objective To investigate the possible association between the allelic variation of LAPTM4B and the genetic susceptibility of lymphoma. Methods The genotype of LAPTM4B was analyzed in 350 unrelated healthy adult individuals and 166 patients with lymphoma by utilizing polymerase chain reaction based on special primers. The genotypical distribution of LAPTM4B was analyzed by X^2 test. Results The allelic frequencies of the ＊ 2 were 34. 94% and 24. 14% in the lymphoma group and the healthy control group respectively, which was significantly different between the two groups（P〈0. 001 ）. The risk of suffering from gastric cancer was increased 1. 475 times in the individuals of the ＊ 1/2 genotype（95% CI：0.994-2.189）and 3. 532 times in the individuals of the ＊ 2/2 genotype of LAPTM4B（95% CI： 1. 802- 6.923） compared with the ＊ 1/2 genotype. No association between the genotypical distribution of LAPTM4B and the clinical information on patients of lymphoma such as age, gender, pathological type, differentiation classification of TNM, lactate dehydrogenase（LDH）,β2-MG and infection of HBV. Conclusion The allele ＊ 2 of LAPTM4B might be the risk factor of lymphoma, which could be associated with genetic susceptibility of lymphoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2007年第4期245-248,共4页
Cancer Research on Prevention and Treatment
基金
北京市自然科学基金重点资助项目(7041003)
