摘要
目的:筛选子宫肌瘤发病相关基因。方法:分别提取10对子宫肌瘤及肌层组织mRNA,用Cy5-dCTP和Cy3-dCTP逆转录标记cDNA探针,然后与含14000条cDNA的表达谱芯片进行杂交,洗片。将所得芯片进行扫描、聚类分析,获得差异基因表达信息。结果:共得到39条表达差异基因,5条表达上调,34条表达下调。表达下调的基因主要与细胞周期、增殖与凋亡、DNA合成与转录及细胞信号转导相关。结论:子宫肌瘤的发生与发展可能与这些基因的表达失调相关。
Objective： To screen some uterine leiomyoma-associated genes. Methods： The mRNA was isolated and purified from uterine leiomyoma and uterine smooth muscle tissue and then were reversely transcribed to cDNA with the Cy5-dCTP and Cy3-dCTP respectively to mark probe. The probes were hybridized to the cDNA microarray which contains a set of 14 000 human genes. After washed, the cDNA microarray was scanned and gene cluster analyzed for the different gene expression information. Results： Thirty-nine differentially expressed genes were gotten, which 5 up-regulation and 34 down-regulation. The down-regulated genes mainly correlated with cell cycle, proliferation, apoptosis, DNA synthesis and transcription, signal transduction. Conclusion： The genesis and development of uterine leiomyoma may be associated with misalignment of the differentially expressed genes.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2007年第4期270-276,共7页
Reproduction and Contraception
基金
本课题为上海市卫生局医学重点学科重大项目课题
关键词
子宫肌瘤
基因表达
表达谱芯片
uterine leiomyoma
gene expression
cDNA microarray
