摘要
AIM： To explore the relationship between acetylation of histone in total chromatin and p21^WAF1 expression regulation in human colorectal carcinoma. METHODS： We analyzed the expression of tumor suppressor gene p21^WAF1 mRNA by RT-PCR or realtime PCR in 33 samples of colorectal cancerous tissue, corresponding para-cancerous tissue and normal colorectal mucosa, and also examined the level of acetylated histone H3 in total chromatin using Western blotting. RESULTS： The expression level of p21^WAF1 mRNA was significantly lower in colorectal cancerous tissue from 33 patients than in para-cancerous tissue and normal colorectal mucosa （2377.95 ± 865.80 vs 3216.58 ± 1149.42 and 3541.61 ± 1433.17 respectively, P 〈 0.01）. In addition, when p21^WAF1 mRNA expression was undectectable or at very low level （50% less than that in adjacent tissue and normal colorectal mucosa） in all tissues, the level of acetylated histone H3 in colorectal cancerous tissue was significantly lower than that in corresponding para-cancerous tissue and normal colorectal mucosa in five of seven （71.43%） cases. The transcriptional level of p21^WAF1 in colorectal carcinoma might not be associated with its biological behaviors.CONCLUSION： The down-regulation of p21^WAF1 transcription is involved in the tumorigenesis and development of colorectal carcinoma. The down-expression of p21^WAF1 mRNA in colorectal carcinoma might be associated with histone hypoacetylation in chromatin but not with biological behaviors.
瞄准：探索在乙酰化作用之间的关系嘘在全部的染色质的和在人的 p21WAF1 表示规定渲染表面的癌。方法：我们分析了肿瘤的表示压制或由 RT-PCR 的基因 p21WAF1 mRNA 或真实 -- 在表面的癌的织物，相应帕拉癌的织物和正常渲染的颜色的 33 件样品的时间 PCR 表面的粘膜，并且也检验了乙酰 ated 的水平嘘在全部的染色质使用西方的弄污的一 H3。结果：p21WAF1 mRNA 的表示水平是显著地更低的在比在帕拉从 33 个病人渲染表面的癌的织物 -- 癌的织物和正常渲染表面的粘膜(2377.95 +/- 865.80 对 3216.58 +/- 1149.42 和 3541.61 +/- 1433.17 分别地， P < 0.01 ) 。另外，当 p21WAF1 mRNA 表示是 undectectable 时或在很低级(50%不到那在邻近的织物和正常渲染表面的粘膜)在所有纸巾，乙酰 ated 的水平嘘在颜色的一 H3 表面的癌的织物在相应帕拉癌的织物是比那显著地低的，正常在七中的五个渲染表面的粘膜(71.43%)盒子。在颜色的 p21WAF1 的 transcriptional 水平表面的癌可能没与它的生物行为被联系。结论：p21WAF1 抄写的下面规定涉及颜色的肿瘤发生和发展表面的癌。在颜色的 p21WAF1 mRNA 的下面表示表面的癌可能与被联系嘘在染色质然而并非与生物行为的一 hypoacetylation。
基金
Supported by the Major State Basic Research Development Program of China (973 Program), No. 2005CB522400
Shanghai Leading Academic Discipline Project, No. Y0205
Shanghai Municipal Commission for Science and Technology to FJY. No.04DZ14006
