摘要
目的研究犬颅脑枪弹伤后脑组织中脑源性神经生长因子(BDNF)和怏反应蛋白c-myc的变化规律。方法24只杂种犬随机分为对照组(n=4),枪弹伤组(n=20)。采用德国小口径步枪子弹致犬颅脑额叶切线伤。用免疫组织化学方法检测伤后0.5、2、6、12和24 h各不同时期弹道挫伤区、震荡区、海马及脑干神经元中BDNF和c-myc蛋白的表达。结果对照组脑神经元中BDNF和c-myc蛋白表达弱。枪弹伤组挫伤区、震荡区、海马及脑干神经元中BDNF和c-myc蛋白表达0.5 h开始增加(P<0.05),2 h达高峰(P<0.01),6 h开始下降,c-myc表达在24 h同对照组,但BDNF表达在24 h仍高于对照组。两者在不同区域、不同时间之间的表达不完全一致。结论BDNF和c-myc在弹道挫伤区、震荡区、海马及脑干神经元均有表达,两者在脑组织中表达的时空变化规律基本一致,可能共同参与细胞抗凋亡过程,相互间也可能存在着一定的调控作用。
Objective To investigate the expressions and changes ofbrain-derivod neurotrophic factor （BDNF） and c-myc in brain following craniocerebral gunshot wounds in dogs. Methods Twenty-four mongrel dogs were randomly divided into control and injury group. The canine models of tangent brain injury （TBI） were established by Germany-made bullets of small-caliber rifles. BDNF and c-myc positive expression in neurons were determined by immunohistochemistry in the trajectory contusion area, the trajectory concussion area, hippocampus and brain stem at 0.5, 2, 6, 12 and 24 h after trauma. Results Weaker expressions of BDNF and c-myc were detected in the control group. Positive expressions of BDNF and c-myc in neurons in the trajectory contusion area, the trajectory concussion area, hippocampus and brain stem increased at 0.5 h （P〈0.05） after craniocerebral gunshot wounds, reached the peak at 2 h （P〈0.01） and began to decrease at 6 h. Positive expression of c-myc had no difference from that of control group at 24 h, but positive expression of BDNF was higher than that of control group at 24 h. The expressions varied in different brain regions at different time. Conclusion The expressions of BDNF and c-myc in the trajectory contusion area, the trajectory concussion area, hippocampus and brain stem are the early reaction to craniocerebral gunshot wounds. The temporal and spatial expression regulation of BDNF might be similar to that of c-myc. BDNF and c-myc may be together associated with anti-apoptosis, and have a regulative function between each other.
出处
《中华神经医学杂志》
CAS
CSCD
2007年第5期468-470,共3页
Chinese Journal of Neuromedicine
基金
全军"九五"医学科研基金重点资助项目(99-Z-146)
