摘要
目的:研究Ⅳ型弥漫增殖型狼疮肾炎、轻微性肾小球病变及正常肾组织中趋化因子fractalkine及其受体CX3CR1的表达,以及与CD68阳性巨噬细胞表达之间的相互关系,探讨fractalkine/CX3CR1在Ⅳ型狼疮肾炎发病中的作用。方法:Ⅳ型狼疮肾炎患者的肾活检组织21例,轻微性肾小球病变患者的肾活检组织18例,光镜下正常的肾组织标本8例。采用免疫组织化学技术检测肾组织中fractalkine、CX3CR1及CD68的表达。结果:(1)正常肾组织和轻微性肾小球病变肾组织中fractalkine基本未见阳性表达;其受体CX3CR1阳性细胞和CD68阳性巨噬细胞仅偶见于肾小球及肾皮质间质中。(2)Ⅳ型狼疮肾炎肾小球和肾皮质间质中CX3CR1阳性细胞和巨噬细胞显著增多,且两者的表达显示高度的相关性(分别为r=0.956,P<0.01和r=0.965,P<0.01)。(3)Ⅳ型狼疮肾炎肾组织中fractalkine高表达于皮质肾小管上,fractalkine阳性皮质肾小管的百分比分别与肾皮质间质CX3CR1阳性细胞数和巨噬细胞数之间高度相关(分别为r=0.720,P<0.01和r=0.770,P<0.01)。结论:提示fractalkine/CX3CR1在Ⅳ型狼疮肾炎的发病机制中可能占有重要地位。
AIM： To observe the expression of fractalkine, and its receptor, CX3CR1, in renal tissues of patients with diffuse proliferative lupus glomerulonephritis （WHO class Ⅳ）, minimal glomerular abnormalities, and normal kidney. Meanwhile, the correlation among the expression of fractalkine, CX3CR1 and CD68 -positive macrophages was investigated, and the role of fractalkine and CX3CR1 in the pathogenesis of lupus nephritis was discussed. METHODS： The expressions of fractalkine, CX3CR1 and CD68 were detected immunohistochemically in kidney tissue sections obtained from twenty - one patients with WHO class Ⅳ lupus nephritis, eighteen cases with minimal glomerular abnormalities, and eight normal kidneys which were no abnormality under light microscope. RESULTS： （ 1 ） Fractalkine was generally indistinguishable in tissue sections from normal kidney and minimal glomerular abnormalities. CX3CR1 -positive cells and CD68 -positive macrophages were sparsely detected in the glomeruli and in the cortical interstitium. （2） There were considerable CX3CR1 - positive cells and macrophages in both the glomeruli and the interstitium in sections from class Ⅳ lupus nephritis. The number of CX3CR1 - positive cells significantly correlated with the number of macrophages in the glomeruli and in the interstitium respectively （ r = 0. 956, P 〈 0. 01 and r = 0. 965, P 〈 0. 01 ）. （3） Significant expression of fractalkine was seen in the cortical renal tubules from class Ⅳ lupus nephritis. The percentage of fractalkine - positive tubules significantly correlated with the number of CX3CR1 - positive cells and macrophages in the interstitium respectively （ r=0. 720, P 〈 0. 01 and r= 0. 770, P 〈 0.01 ）. CONCLUSION： These expression patterns show that fractalkine and CX3CR1 may play an important role in the pathogenesis of class Ⅳ lupus nephritis.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2007年第5期908-912,共5页
Chinese Journal of Pathophysiology
基金
教育部博士点基金资助项目(No.2000056916)
