摘要
目的:研究NF-κBdecoy寡核苷酸对LPS诱导结肠癌细胞SW480后,对TLR4、IL-8表达的影响。方法:体外培养SW480细胞,用LPS(10μg/L)刺激3h后,用脂质体lipofectin2000介导NF-κBdecoyODNs转染6h,收集细胞上清液,用ELISA法检测IL-8;提取细胞mRNA,经RT-PCR法检测TLR4 mRNA、IL-8 mRNA的表达。并设对照组、ScrambledODNs组、lipofectin2000组进行比较。结果:LPS刺激后TLR4 mRNA、IL-8 mRNA和IL-8的表达明显强于对照组;用NF-κBdecoy寡核苷酸干预,可以明显抑制TLR4 mRNA、IL-8 mRNA和IL-8的表达。而ScrambledODNs组和转染剂lipofectin2000组对其没有明显影响。结论:NF-κBdecoyODNs有望成为治疗IBD的一种新型基因药品。
AIM： To study the effect of NF - κB decoy oligodeoxynucleotides （ODNs） on TLR4 and IL -8 expression in LPS - induced SW480 cells. METHODS： SW480 cells were cultured in vitro and stimulated for 3 h with LPS （10 μg/L）. NF- κB decoy oligodeoxynucleotides mediated by lipofectin 2000 were added into the cell culture for 6 h. The supernatants were collected and messured for IL -8 by ELISA. TLR4 mRNA and IL- 8 mRNA were examined by RT -PCR, respectively. The results were compared with control group, Scrambled ODNs group and lipofectin 2000 group. RESULTS： After SW480 cells were stimulated by LPS, TLR4 mRNA, IL- 8 mRNA and IL- 8 expressions were signifi- candy increased, and the difference compared with control group was obvious. After treated with NF - κB decoy oligodeoxy- nucleotides, TLR4 mRNA, IL- 8 mRNA and IL- 8 expressions were significantly inhibited. The Scrambled ODNs group and lipofectin 2000 group had no effect on them. CONCLUSION： NF - κB decoy ODNs will become a new gene drug for treating inflammatory bowel disease（IBD）.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2007年第5期934-938,共5页
Chinese Journal of Pathophysiology
基金
河北医科大学第二医院科研基金资助项目(No.2h1200606)
