摘要
目的研究猪骨髓间充质干细胞成骨分化后(osteogenic cells differentiatedfrommesenchymal stemcell,DOC)在IFN-γ刺激下猪白细胞抗原(swine lymphocyte antigen,SLA)基因的表达变化及对外周单个核细胞(PBMC)增殖的影响。从而了解其免疫原性。方法以未分化的MSC为对照,采用RT-PCR技术分别检测DOC、未分化的MSC、MSC+IFN-γ组、DOC+IFN-γ的SLA基因表达情况,采用单向混合淋巴反应观察各组细胞对植物血凝素(PHA)刺激PBMC的每分钟脉冲值(CPM)变化。结果DOC组、MSC+IFN-γ组、DOC+IFN-γ组SLA-I(P1,P14)表达上调(P<0.05),SLA-Ⅱ(DRA、DRB、DQA、DQB)表达明显上调(P<0.01)。并能抑制PHA刺激的PBMC增殖反应。结论在体外实验中,DOC在IFN-γ刺激下仍具有低免疫原性,且具有免疫调节作用。
Objective To investigate the gene expression variation of swine lymphocyte antigen （SLA） on esteogenie cells differentiated from mesenchymal stem cells （DOC） under stimulation of IFN-γ, and its effects on proliferation of peripheral blood mononuclear cell （PBMC）, for explaining the immunogenicity of SLA. Methods The SLA gene expression variation of DOC treated with or without IFN-γ was detected by RT-PCR, with undiferentiated MSC as control group. CPM variation of PBMC ＋ phytohemagglutinin （PHA） was explored by one-aymixed lymphocyte culture （MLC） after adding DOC treated with or without IFN-γ. Results Porcine SLA-Ⅰ（P1, P14）expressions were up-regulated in DOC, MSC ＋ IFN-γ, and DOC ＋ IFN-γ groups （P 〈0.05）, while SLA- Ⅱ （DRA, DRB, DQA, and DQB） were up-regulated significantly in these groups （ P 〈 0.01 ）. DOC could suppress PBMC proliferation induced by PHA. Conclusion In vitro, whatever DOC treated with IFN-γ or not, DOC still keep low immunogenieity and perform immunogenie function.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2007年第3期298-301,305,共5页
Immunological Journal
基金
国家高技术研究发展资助重大攻关课题资助("863"计划
2006-AA205020)
关键词
骨髓间充质干细胞
成骨分化
SLA
免疫原性
Mesenehymal stem cell
Osteogenie cells differentiated from mesenehymal stem cells
Swine lymphocyte antigen
Immunngenicity
