期刊文献+

榄香烯治疗人喉癌裸鼠模型的实验研究 被引量:8

Elemene in the treatment of the transplantable model of human laryngeal squamous carcinoma in nude mice
原文传递
导出
摘要 目的:研究榄香烯对人喉鳞状细胞癌细胞株Hep-2细胞荷瘤裸鼠模型移植瘤的生长抑制作用,探讨其抑瘤机制。方法:建立人喉鳞状细胞癌Hep-2细胞株裸鼠皮下移植模型。应用榄香烯治疗,观察移植肿瘤和裸鼠生长情况,计算肿瘤抑制率;逆转录PCR检测肿瘤组织中血管内皮生长因子C(VEGF-C)和血管内皮生长因子受体3(VEGFR-3)mRNA表达情况。结果:榄香烯对人喉鳞状细胞癌细胞株Hep-2细胞荷瘤裸鼠模型移植瘤的增殖具有明显抑制作用,抑瘤率为52.24%。在实验组和对照组之间,鼠净重、瘤重和瘤体积相比,差异有统计学意义(均P<0.01);实验组VEGF-C和VEGFR-3mRNA的表达低于对照组(P<0.01)。结论:榄香烯可显著抑制人喉鳞状细胞癌裸鼠模型肿瘤的生长,其机制与榄香烯抑制VEGF-C和VEGFR-3的表达有关。 Objective:To investigate the inhibitory effect of elemene on the tumor growth of human laryngeal squamous carcinoma (LSCC) and to explore the possible mechanism of its inhibitory effect on LSCC. Method: Nude mice model engrafted with laryngocarcinoma was established by using human laryngeal squamous carcinoma cell line (Hep-2). When the transplantable tumor size is more than 10 mm, the mice were given elemene (120 mg/kg) in the treatment group or PBS in the control group , at the day of 1、4、7、10、13、16、19 and 22 respectively. The animals were killed three days after the last treatment. The tumor volume, tumor weight,mice net weight and tumor inhibition rate were measured. The expression of vascular endothelial growth factor- C (VEGF- C) and vascular endothelial growth factor receptor- 3 (VEGFR- 3) mRNA were quantitated by using reverse transcription and polymerase chain reaction technique. Result: Elemene could inhibit the tumor growth in vivo. The differences were statistically significant for the mice net weight, tumor weight, and tumor volume between the treatment group and the control group. The tumor inhibition percentage was 52.24%. The gene expression of VEGF-C and VEGFR-3 of the treatment group is lower than that of the control group and the differences were statistically significant ( P 〈0.01). Conclusion: Elemene could inhibit the subcutaneous plantation of human laryngeal carcinoma in nude mice and its mechanism may be associated with inhibited expression of VEGF-C and VEGFR-3.
出处 《临床耳鼻咽喉头颈外科杂志》 CAS CSCD 北大核心 2007年第9期417-420,共4页 Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词 榄香烯 喉肿瘤 鳞状细胞 血管内皮生长因子C 血管内皮生长因子受体3 Elemene Laryngeal neoplasms Carcinoma,squamous cell Vascular endothelial growth factor-C Vascular endothelial growth factor receptor-3
  • 相关文献

参考文献9

  • 1AMIOKA T,KITADAI Y,TANAKA S,et al.Vascular endothelial growth factor-C expression predicts lymph node metastasis of human gastric carcinomas invading the submucosa[J].Eur J Cancer,2002,38:1413-1419.
  • 2陶磊,周梁,郑璐滢,姚明.榄香烯对真核细胞翻译起始因子家族表达和血管生成的抑制作用[J].中华耳鼻咽喉头颈外科杂志,2005,40(11):840-845. 被引量:27
  • 3曾宗渊,陈福进,张诠,杨安奎,曾祥发,陈文宽,郭朱明,许光普.手术加放射治疗与单纯喉部分切除术治疗喉癌的疗效比较[J].中华耳鼻咽喉科杂志,2000,35(1):32-34. 被引量:86
  • 4汪志明,罗奋,陈浩.榄香烯乳淋巴吸收途径对进展期胃癌淋巴转移的作用[J].中国肿瘤临床,2004,31(24):1396-1400. 被引量:6
  • 5LI X,WANG G,ZHAO J,et al.Antiproliferative effect of beta-elemene in chemoresistant ovarian carcinoma cells is mediated through arrest of the cell cycle at theG2-M phase[J].Cell Mo1 Life Sci,2005,62:894-904.
  • 6FRANCHI A,MASSI D,SANTUCC M I,et al.Inducible nitric oxide synthase activity correlates with lymphangiogenesis and vascular endothelial growth factor-C expression in head and neck squamous cell carcinoma[J].J Pathol,2006,208:439-445.
  • 7JENNBACKEN K,VALLBO C,WANG W,et al.Expression of vascular endothelial growth factor C (VEGF-C) and VEGF receptor-3 in human prostate cancer is associated with regional lymph node metastasis[J].Prostate,2005,65:110-116.
  • 8SHIMIZU K,KUBO H,YAMAGUCHI K,et al.Suppression of VEGFR-3 signaling inhibits lymph node metastasis in gastric cancer[J].Cancer Sci,2004,95:328-333.
  • 9李胜,许有威,任东文,郑建华,马小军.β-榄香烯海藻酸钙-壳聚糖微囊的制备[J].中国医药工业杂志,2004,35(11):657-660. 被引量:21

二级参考文献22

共引文献135

同被引文献150

引证文献8

二级引证文献142

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部