摘要
目的观察肝苏颗粒对实验性黄疸大鼠的保护作用及其机制的初步探讨。方法采用α-萘异硫氰酸酯(ANIT)灌胃制备大鼠黄疸模型,共60只大鼠,设正常组、模型组、熊去氧胆酸(UDCA)组、肝苏颗粒小剂量组和肝苏颗粒大剂量组,检测不同组血清ALT、TBil和一氧化氮(NO)、IL-6的变化,流式细胞仪检测肝细胞凋亡,免疫组化检测肝细胞Bcl-2、Bax蛋白的表达。结果肝苏颗粒大、小剂量治疗组和UDCA组与模型组比较,ALT及TBil降低明显,ALT分别为(547.20±79.41)U/L、(573.34±68.79)U/L、(535.60±69.39)U/L、(642.30±92.70)U/L,TBil分别为(47.10±11.54)μmol/L、(50.87±13.46)μmol/L、(45.22±14.46)μmol/L、(66.19±10.67)μmol/L,差异有统计学意义(P<0.05或P<0.01)。肝苏颗粒治疗组和UDCA组NO、IL-6水平与模型组比较下降显著,差异有统计学意义(P<0.01或P<0.05)。肝苏颗粒治疗组、UDCA组与模型组比较,肝细胞凋亡率下降,差异有统计学意义(P<0.01或P<0.05);肝苏颗粒大剂量治疗组、UDCA组Bcl-2和Bax阳性表达及Bax/Bcl-2相对比率明显低于模型组,差异有统计学意义(P<0.01或P<0.05)。结论肝苏颗粒对实验性黄疸大鼠的肝功能具保护作用,影响血清NO、IL-6及抑制肝细胞凋亡可能是其退黄、恢复肝脏功能的作用机制之一。
Objective To study the effect and mechanisms of gansu capsule in experimental icteritious rats. Methods The experimental icteritious model was made by per/using alpha-naphthyli- sothiocyanate （ANIT） by mouth in Wistar rats. Normal control group, experimental icteritious model group, ursodeoxycholic acid （UDCA） group, gansu capsule low dose group and gansu capsule high dose group were set up. The levels of alanine aminotransferase （ALT）, total bilirubin （TBil）, nitric oxide （NO）, interleukin-6 （IL-6） in blood plasma of the five groups were examined. The rates of apoptosis of hepatocytes were analysed by flow cytometry. The expression of Bax and Bcl-2 was detected qualitatively and quantitatively by immunohistochemistry assay using an image analysis system. Results The levels of ALT and TBil in the gansu capsule treatment group （large dose group and small dose group） and UDCA group were lower than those of the experimental icteritious model group. ALT was （547.20 ± 79.41） U/L, （573.34 ±68.79） U/L, （535.60 ± 69.39） U/L, （642.30 ±92.70） U/L respectively and TBil was （47.10± 11.54） μ/mol/L, （50.87 ± 13.46） μmol/L, （45.22 ±14.46）μmol/L, （66.19 ±10.67） μmol/L respectively. The differences between the gansu capsule groups or UDCA group and the model group were significant （P 〈0.01 or P 〈 0.05）. The levels of NO and IL-6 in the gansu capsule groups and the UDCA group were lower than those of the model group, and the differences were significant （P 〈 0.01 or P 〈 0.05） . The rates of hepatocytes apoptosis of the gansu capsule treatment groups or the UDCA group were lower than that of the model group, and the differences were significant （P 〈 0.01 or P 〈0.05）. The comparing ratios of Bax and Bcl-2 proteins （Bax/Bcl-2） expressed in hepatocytes in gansu capsule high dose group and UDCA group were lower than that of the model group, the differences were significant （P 〈 0.01 or P 〈 0. 05）. Conclusion Gansu capsule influence secretion of NO and IL-6 level in plasma and inhibit hepatocytes apoptosis to protect liver.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2007年第3期143-146,共4页
Chinese Journal of Infectious Diseases
