摘要
目的阐明结核分枝杆菌耐异烟肼临床分离株katG基因突变特点以及Spoligotyping分型和结核病流行关系。方法对123株结核分枝杆菌临床分离株,其中有药敏结果的98株,(耐异烟肼菌株45株;异烟肼敏感株53株)的katG基因进行DNA片断扩增然后进行SSCP分析;同时对其中的121株菌株进行Spoligotyping分型。结果45株耐INH结核杆菌株中,27株(60%)第315位点突变,低耐药菌株(1mg/L)第315位点突变率显著高于高耐药菌株(10mg/L;53株敏感株中无KatG基因315位密码子突变。对121株临床株的SpoligotypingDNA指纹分析,1型,(北京型)103株占84%(103/121),其它基因型18株,分散在13种基因型中。结论本项研究进一步证实了结核分枝杆菌耐异烟肼与katG基因突变之间的关系;北京型感染是北京地区结核病流行的重要原因。
Objective To study katG gene mutations and molecular epidemiological distribution characteristics in isoniazid resistant clinical isolates of Mycobacterium tuberculosis. Methods A total of 123 strains of Mycobacterium tuberculosis were analyzed by PCR- SSCP and DR-based Spoligotyping DNA fingerprinting. Results Point mutations at position 315 were found in the genomes of 60% （27/ 45 ）of isoniazid resistant strains. Low level isoniazid resistant strains （ 1 mg/L） had higher mutation frequency at position 315 than high level isoniazid resistant strains （ 10 mg/L;χ^2 =9.31 ,P 〈0. 05）. No mutations were identified in the katG gene at position 315 in 53 isoniazid susceptible isolates. 101 of 121 M tuberculosis strains from this survey were determined to be ＂Beijing Genotype＂ M tuberculosis strain by DNA fingerprinting analysis, and 18 of 121 strains were scattered in 13 kinds of the other Genotypes. Conclusion These studies provide further evidence supporting the association between katG, gene mutations and INH resistance in Mycobacterium tuberculosis; M tuberculosis strains of Beijing Genotype are prevailing in Beijing district at present.
出处
《临床肺科杂志》
2007年第6期567-569,共3页
Journal of Clinical Pulmonary Medicine
