摘要
目的:研究大鼠脊髓渐进性压迫减压后修复过程中神经细胞黏附分子(NCAM)的表达情况.方法:Wistar大鼠51只,分为空白对照组(n=3)和损伤组(n=48).空白对照组不做任何处理,常规饲养;损伤组用螺钉压迫法造成脊髓渐进性压迫模型,压迫至中度开始减压.按减压后不同时间点又分为1,3,5,7,14和28d6个组,每组动物8只.用免疫组织化学SABC法测定NCAM在脊髓灰质神经元中的表达,并采用计算机Mias软件进行图像分析.结果:各组灰度值:空白对照组:245.53±3.47;损伤组减压后1d组:235.33±6.48;3d组:218.86±2.67;5d组:198.39±3.24;7d组:173.15±5.98;14d组:190.45±4.93;28d组:227.68±2.85.与空白对照组相比脊髓渐进性损伤组各不同时间点组均有NCAM的表达,差异具有统计学意义(P<0.01),其中以减压后7dNCAM的表达量最高.结论:在脊髓渐进性压迫减压后的修复过程中NCAM均有表达,NCAM表达量的变化可能与脊髓损伤后的修复有关.
AIM： To investigate the expression of neural cell adhesion molecule （NCAM） in the neural repair after spinal cord compressive injury in rats. METHODS： Fifty-one adult Wistar rats were randomly divided into 2 groups ： control group （ n = 3 ） , injury group （ n =48 ）. The control group were raised normally without any treatment and the injury group were gradual compressive injury of spinal cord by bolt compressing. The injury group was again divided into 6 subgroups （ n = 8/subgroup） according to the time after gradual decompression （ 1,3, 5, 7, 14 and 28 d）. The expression level of NCAM in spinal cord at all the time points was observed by SABC methods of immunohistochemistry and analyzed by Mias image analysis software. RESULTS： The grayscale value of every group is 245.54 ± 3.47 （ control group） and 235.33 ±6.48 （injury group 1 d）, 218.86 ±2.67 （ injury group 3 d）, 198.39±3.24 （injury group 5 d）, 173.15 ±5.98 （injury group 7 d）, 190.45 ±4.93 （injury group 14 d）, 227.68 2.85 （ injury group 28 d）. NCAM was expressed in each subgroups of injury group with the statistical difference as compared with control group （P 〈 0.01 ）, and at the 7th day after gradual decompression immunopositive reaction of NCAM was the strongest among them. CONCLUSION： NCAM expresses in the course of repair after spinal cord injury and the density of expression may be considered as a molecular evidence of neural repair.
出处
《第四军医大学学报》
北大核心
2007年第9期822-825,共4页
Journal of the Fourth Military Medical University
关键词
神经细胞黏附分子
脊髓损伤
neural cell adhesion molecule
spinal cord injuries
