摘要
Using microsatellite markers with 10 pairs of primers,genetic structure of Oreochromis niloticus,Sarotherodon melanotheron and their hybrids were analyzed.The results suggested that:① Six pairs of primers showed a rich polymorphic,which can be used as genetic markers for the genetic diversity study of these tilapias.② The analysis based on the six pairs of primers showed that the average heterozygosity(H)ranged as:O.niloticus ♀× S.melanotheron♂(0.725),S.melanotheron ♀ × O.niloticus ♂(0.692),S.melanotheron(0.537),O.niloticus(0.485).The highest effective numbers of alleles(Ne)existed in O.niloticus♀×S.melanotheron♂(3.79),the lowest existed in S.melanotheron(1.92).The average polymorphism information content(PIC)of 4 tilapias was 0.584,the rank from high to low as,O.niloticus ♀ × S.melanotheron ♂(0.668),S.melanotheron ♀× O.niloticus ♂(0.643),O.niloticus(0.375),S.melanotheron(0.333).③ The fact of enhanced heterozigosity of hybrids suggested that there were the genetic basement of cross breeding in tilapia.
Using microsatellite markers with 10 pairs of primers, genetic structure of Oreochromis niloticus, Sarotherodon melanotheron and their hybrids were analyzed. The results suggested that： O Six pairs of primers showed a rich polymorphic, which can be used as genetic markers for the genetic diversity study of these tilapias. ② The analysis based on the six pairs of primers showed that the average heterozygosity（H） ranged as： O. niloticus ♀×S . melanotheron♂ （0. 725）, S . melanotheron ♀×O . niloticus ♂ （0. 692）, S . melanotheron （0. 537）, O. niloticus （0. 485）. The highest effective numbers of alleles（Are） existed in O. niloticus ♀× S. melanotheron♂ （3.79）, the lowest existed in S. melanotheron （1.92）. The average polymorphism information content （PIC） of 4 tilapias was 0.584, the rank from high to low as, O. niloticus ♀× S. melartotheron♂ （0.668）,S. melanotheron ♀× O. niloticus ♂ （0.643）,0. niloticus （0.375）,S. melanotheron （0.333）. （3） The fact of enhanced heterozigosity of hybrids suggested that there were the genetic basement of cross breeding in tilapia.
出处
《水产学报》
CAS
CSCD
北大核心
2007年第3期411-415,共5页
Journal of Fisheries of China
基金
国家"十五"攻关项目--尼罗罗非鱼选育技术(2001BA505B0513)
关键词
尼罗罗非鱼
萨罗罗非鱼
杂交后代
基因组DNA
微卫星标记
Oreochrotnis niloticus
Sarotherodon melanotheron
hybrids
genomic DNA
micmsatellite markers Oreochrotnis niloticus
Sarotherodon melanotheron
hybrids
genomic DNA
micmsatellite markers
