摘要
采用单因素试验对甘草地上部分(茎叶)的超临界CO2提取工艺进行了研究。实验考察了压力、萃取时间、温度及CO2流量对甘草地上部分总黄酮提取率的影响,以总黄酮提取率和含量为指标,系统的研究了超临界二氧化碳萃取法提取甘草地上部分总黄酮的提取效果。得出的最佳工艺参数为:采用40-60目原料,80%乙醇为夹带剂,萃取时间:1.5 h;萃取压力:30.0 MPa;萃取温度:50℃;CO2流量:10 kg.h-1;分离压力:5.8 MPa;分离温度:40℃。实验结果表明超临界二氧化碳萃取甘草总黄酮的提取率2.09%,含量5.42%,工艺具有提取率高,纯度高的特点,为规模化生产甘草总黄酮的提取提供了研究基础。
Factors of effect on the yield of flavonoids from licorice of overground stem and leaves were studied by single factor＇ s analysis experiments. The single factor＇ s analysis experiments were carried out to investigate influential factors of extraction temperature, pressure, flux of CO2 and extraction time. The extraction of flavonoids with Supercritical CO2 was studied on the yield of extracts. It had been found that the optimized technological parameters were： particle size 40 - 60 sieve mesh, 80% ethanol as entrainer, extraction time 1.5 h, extraction pressure 30 MPa, extraction temperature 50℃, CO2 flow rate 10 kg · h^-1, separation pressure 5, 8 MPa, separation temperature 40℃. The yield of flavonoids was 2.09% and the content was 5.42%. The results offered basic research of a new method for the product of flavonoids of licorice.
出处
《植物研究》
CAS
CSCD
北大核心
2007年第3期372-375,共4页
Bulletin of Botanical Research
基金
教育部新世纪优秀人才支持计划(NCET-04-0316)
哈尔滨市科技攻关计划项目(2004AA3CS172-2)
黑龙江省自然科学基金项目(ZJN04-03)
关键词
甘草
总黄酮
超临界CO2萃取
Licorice
flavonoids
Supercritical CO2 Extraction
