摘要
目的:研究胃癌组织中PTEN(phos-phatase and tensin homologne derived from chromosome ten)和血管内皮生长因子(vas-cular endothelial growth factor)的表达、相关性及其临床意义。方法:应用SABC免疫组织化学方法检测108例胃癌和64例胃良性病变中PTEN和VEGF的表达状况。结果:PTEN在胃癌和胃良性病变中的阳性表达率分别为45.4%(49/108)和87.5%(56/64)(χ2=29.99;P<0.001),VEGF在胃癌和胃良性病变中的阳性率分别为77.8%(84/108)和7.8%(5/64)(χ2=78.78;P<0.001);PTEN阴性胃癌中VEGF阳性表达率为59.2%(29/49),明显低于PTEN(-)VEGF阴性胃癌93.2%(55/59)(χ2=17.94;P<0.001)。PTEN阳性表达与胃癌Lanren分型、浸润深度、淋巴结转移及PTNM分期有关,P<0.01;VEGF与胃癌浸润深度、淋巴结转移及PTNM分期有关,P<0.01。结论:胃癌中存在PTEN下调和VEGF上调,且两者具有负相关;二者的表达水平可作为判定胃癌病理生物学行为的客观指标。
OBJECTIVE： To study the correlation of phos phatase and tensin homologne derived from chromosome ten （PTEN） protein expression and vascular endothelial growth fac tor （VEGF） expression in gastric carcinoma （GC） and the clinical significance. METHODS： PTEN protein and VEGT in 108 cases of GC and 64 cases of gastric benign disease （GBI）） were detected with SABC immunohistochemical method. RESULTS： The posi tive rate of expression of PTEN protein in GC and GBI） were 45.4%（49/108） and 87. 5% （56/64）, respectively （X^2= 29.99, P〈0. 001）. The total positive rate of expression of VEGF in GC and GBD were 77.8% （84/108） and 7.8%（5/64） respectively （X^2= 78.78; P〈0. 001）. In PTEN positive GC cases, the positive rate of VEGF （59.2% was significantly lower than than of PTEN negative GC cases （93.2% , X^2 = 17.94, P〈0. 001 ） ; The expression of PTEN in GC was significanfly correlated with the histological type, invasion depth, lymph node metastasis and TNM stage, P〈0. 01. while the expression of VEGF in GC cases was significantly associated with the invasion depth, lymph node metastasis and TNM stage, P 〈 0.01. CONCLUSIONS： There is a significant negative correlation between PTEN protein expression and VEGF expression in GC. PTEN may play a key role to determine biological behavior of gastric carcinoma.
出处
《中华肿瘤防治杂志》
CAS
2007年第8期601-603,共3页
Chinese Journal of Cancer Prevention and Treatment
