摘要
目的通过体外实验,观察11种化学治疗药物对人卵巢癌细胞的杀伤作用,及其对外周血有核细胞(白细胞、淋巴细胞和粒细胞)的毒性效应,并探讨这2种作用可能存在的相关性。方法采用经典的MTT比色法分别检测34例卵巢癌患者新鲜肿瘤组织标本的癌细胞对11种化疗药物的敏感性和药物对外周血有核细胞的毒性。结果各药物对癌细胞抑制率的差异有显著性;其中,TAX和VM-262种化疗药物的抑制率分别达到50.97%和41.38%,可认为属于卵巢癌细胞的敏感药物;其余9种药物对卵巢癌细胞的抑制率均较低。各药物对癌细胞的抑制率与其对有核细胞中的粒细胞的毒性作用有明显的相关性。结论TAX和VM-26,属于卵巢癌细胞敏感的化疗药物;检测的11种化疗药物,其对卵巢癌的疗效与其对粒细胞的毒性显著相关;在缺乏进行肿瘤细胞药敏试验条件的情况下,卵巢癌患者外周血粒细胞毒性试验,是1种可行的替代检测措施。
Objective To study the killing effect of 11 cytotoxic drugs on the ovarian cancer cells and on the peripheral blood nucleated cells （ leucocytes, lymphocytes and granulocytes）, and to investigate the relationship between the two effects. Methods The sensitivity of 11 cytotoxic drugs on the tumor cells of 34 cases with ovarian cancer and on the peripheral blood nucleated cells were tested in vitro by the classic MTF colorimetric assay. Results there were statistical differences of inhibition ratio of the above-mentioned drugs on the tumor cells. The inhibition ratio of the TAX and VM-26 on tumor cells were 50.97% and 41.38% respectively. They were regarded as chemosensitive to the ovarian cancer cells. The inhibition ratios of the other drugs on the tumor cells were lower. The killing effects of the drugs on the tumor cells were correlated to their effects on the peripheral blood nucleated cells. Conclusion The ovarian cancer cells are sensitive to TAX and VM-26. The killing effects of the drugs on the tumor cells are strongly correlated to their effects on the peripheral blood nucleated cells. These results suggest that the study of the chemo-sensitivity of the peripheral blood nucleated cells could replace the study on the tumor cells in choosing chemotherapeutic drugs in the situation that the study on the tumor cells is not possible.
出处
《实用癌症杂志》
2007年第3期298-301,共4页
The Practical Journal of Cancer
基金
广西科学研究与技术开发计划项目基金资助(桂科攻023502413)
关键词
卵巢癌
细胞毒性
化学治疗
药物敏感试验
Ovarian Cancer
Cytotoxicity
Chemotherapy
Chemosensitivity test
