摘要
目的观察体外培养的骨髓基质细胞与珊瑚羟基磷灰石复合后的生长特性,论证珊瑚羟磷灰石作为骨组织工程学载体材料的可行性。方法分离纯化的狗骨髓基质细胞与珊瑚羟基磷灰石复合体体外培养,分别在相差显微镜下、扫描电镜下观察细胞的表面形态结构。结果骨髓基质细胞在珊瑚羟基磷灰石上贴附生长良好,功能正常。结论骨髓基质细胞在珊瑚羟基磷灰石上生长增殖良好,珊瑚羟磷灰石是骨组织工程载体材料的可靠选择。
Objective To observe the ultrastructure of bone marrow stromal cells （BMSCs） cultured in comlline hydroxyapatite （CHA） and evaluate their biocompatibility. Methods BMSCs isolated from dogs were cultured with CHA as the scaffold, and the morphologies of the cells were observed with phase-contrast microscope and scanning electron microscope. Results and Conclusion BMSCs grew well with good attachment to the CHA scaffold and performed normal function, demonstrating CHA as one of useful biocarrier materials for bone tissue engineering.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第5期705-707,共3页
Journal of Southern Medical University
基金
全军十五重点指令性课题(01L075)分题
广东省科技计划项目(C30704)~~
关键词
骨髓基质细胞
珊瑚羟基磷灰石
超微结构
bone marrow stromall cells
coralline hydroxyapatite
ultrastructure
