摘要
以不结球白菜品种暑绿的112个双单倍体(double haploid,DH)株系构成的群体作为作图群体,利用SRAP、SSR、RAPD和ISSR 4种分子标记来构建不结球白菜分子遗传连锁图谱。通过Mapmaker 3.0/EXP软件分析,得到1张不结球白菜分子遗传图谱,图谱总长度1 116.9 cM,共包括14个连锁群,186个多态性分子标记,其中包括114个SRAP、33个SSR、24个RAPD和15个ISSR标记,其中偏分离标记44个,占23.7%。每条连锁群上的标记数在4-27个之间,连锁群的长度在30.3-165.8 cM的范围内,平均图距在3.4-11.1 cM之间,总平均距离6.0 cM。
The first genetic linkage map of non-heading Chinese cabbage （Brassica campestris ssp. chinensis） was constructed, which was based on a DH （doubled haploid） population including 112 DH lines, derived from a released F1 hybrid variety Shulu. Four kinds of markers of SRAP, SSR, RAPD and ISSR were adopted and 186 polymorphic markers including 114 SRAP, 33 SSR, 24 RAPD and 15 ISSR markers were integrated into the resulted map using Mapmaker 3.0/EXP version, which was consisted of 14 linkage groups and covered 1 116.9 cM. A total of 44 （23.7%） distorted markers distributed in the map. Number of markers included in the linkage groups varied from 4 to 27, the length of linkage groups from 30. 3 cM to 165. 8 cM and mean marker interval distance from 3.4 cM to 11.1 cM individually, and a mean marker interval distance of 6. 0 cM between markers.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2007年第2期23-28,共6页
Journal of Nanjing Agricultural University
基金
国家863计划项目(2006AA10Z1C9)
教育部科学技术研究重点项目(106092)
南京农业大学作物遗传与种质创新国家重点实验室开放课题(ZW2004011)
关键词
不结球白菜
DH群体
分子标记
遗传图谱
non-heading Chinese cabbage
doubled haploid population
molecular marker
genetic map
