摘要
目的观察维生素A（VA）对碘过量小鼠肝脏胰岛素样生长因子-I（IGF—I）表达的影响。方法昆明种小鼠按体质量随机分成6组：对照组（含碘50μg/L，VA4000U／kg）；高碘组（含碘3000μg/L，VA4000U／kg）；常碘低VA组（含碘50μg／L，VA0U／kg）；高碘低VA组（含碘3000μg／L，VA0U／kg）；高碘补VAI组（含碘3000μg／L，VA8000U／kg）；高碘补VA2组（含碘3000μg／L，VA16000U／kg）。喂养3、6个月时，ELISA法检测小鼠血清IGF—I水平，RT-PCR法检测肝脏IGF—I mRNA表达水平。结果与对照组相比，3个月时各组血清IGF—I水平未见明显改变．6个月时高碘组、常碘低VA组、高碘低VA组血清IGF—I水平明显下降（q=3．52、3．29、3．84，P〈0．05），高碘补VA组，IGF-I水平有所升高，介于对照组和高碘组之间。3、6个月时，高碘和常碘低VA组肝脏IGF-I mRNA表达水平，与对照组相比，均有降低趋势；3个月时高碘补VA组与高碘组比较，肝脏IGF—I mRNA表达有升高趋势，而6个月时，则未见明显改变，经检验各组差异均无统计学意义（P〉0．05）。结论高碘摄入抑制小鼠肝脏合成分泌IGF—I，补充VA可以部分拮抗高碘的抑制作用。
Objective To observe the influence of vitamin A （VA） on liver Insulin like Growth Factors 1 （IGF-1） expression of iodine excess mice. Methods Kunming mice were randomly divided into six groups： normal （I- 50 μgg/L,VA 4000 U/kg）, HI（I- 3000 μgg/L,VA 4000 U/kg）, low VA（I- 50 μgg/L,VA 0 U/kg）, HI ＋ low VA（I- 3000 μgg/L,VA0 U/kg）, HI ＋ VAI （I- 3000 μgg/L,VA 8000 U/kg） and HI ＋ VA2（I- 3000 μgg/L,VA 16 000 U/kg） group. ELISA was used to measure the concentration of serum IGF-I and RT-PCR to measure the expression level of liver IGF-1 mRNA 3 and 6 months afterwards. Results In the 6th months, the serum IGF-I level of HI, low VA and HI ＋ low VA group decreased obviously compared with that of the normal group（q = 3.52,3.29,3.84,P 〈 0.05）, VA supplement increased the level of IGF-1 to some extent between normal and HI group. Liver IGF-1 mRNA expression level of HI and low VA group showed a decreasing tendency compared with that of the normal grouμg while it increased in HI ＋ VA groups in the period of the first 3 months, but kept unchanged later on. Conclusions The iodine excess inhibits the liver secreting IGF-1 in mice, which is partly antagonized by VA supplement.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2007年第3期249-251,共3页
Chinese Jouranl of Endemiology
基金
国家自然科学基金项目（30671817）
黑龙江省自然科学基金（D0315）
