摘要
用DEAE-52阴离子交换柱提取、纯化牡蛎糖蛋白,分离纯化的牡蛎糖蛋白命名为糖蛋白GⅠ,糖蛋白GⅡ。用葡聚糖凝胶柱层析分别分析纯化糖蛋白GⅠ,糖蛋白GⅡ,结果表明:牡蛎糖蛋白GⅠ中分离出1种糖蛋白组分GⅠ-1;牡蛎糖蛋白GⅡ纯化出4种糖蛋白组分(GⅡ-1,GⅡ-2,GⅡ-3,GⅡ-4),GⅠ-1为未经洗脱的透过峰,该组分可能是糖蛋白凝聚体,由凝胶电泳分析估算得知GⅠ-1,GⅡ-1,GⅡ-2,GⅡ-3的分子量分别为42.5kDa,100.0kDa,55.3kDa,41.4kDa。
Oyster glycoprotein was isolated by DEAE-cellulose and the findings showed that there were two peaks appeared as glycoproteins, named as G Ⅰ and G Ⅱ. Further purification was carried out by SephadexG-200 and only one peak showed for G Ⅰ, named as G Ⅰ-1 and appeared before the elute solution was applied. It was suggested that the peak is probably coagulate of glycoproteins. Four peaks appeared for GⅡ , named as GⅡ-1,GⅡ-2,GⅡ-3,GⅡ-4. The molecular weights of glycoproteins were determined by SDS-PAGE, 42.SkDa,100.0kDa, 55.3kDa,41.4kDa for G Ⅰ-1, GⅡ-1,GⅡ-2,GⅡ-3 respectively.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2007年第2期211-214,共4页
Journal of Shenyang Agricultural University
关键词
牡蛎糖蛋白
分离纯化
分子量测定
oyster glycoprotein
isolation and purification
determination of the molecular weight
