摘要
目的:对比高温高压处理前后纳米氢氧化铝佐剂的理化性质变化,评价高温高压能否用于纳米氢氧化铝佐剂的前期处理。方法:通过测定浊度、pH值和粒径对比121℃30min高压灭菌锅处理纳米氢氧化铝前后的变化。结果pH值在经过高温高压处理后有轻微的降低,浊度、粒径大小与处理前差异不显著,粒径分布没有较大的变化。结论:首次报道纳米化氢氧化铝佐剂在高温高压灭菌前后的理化性质变化,证实纳米氢氧化铝佐剂在疫苗的制备过程中可以使用121℃30min措施进行灭菌处理。
Objective To study the physical and chemical attributes of nanoparticulated aluminum hydroxide adjuvant （NAHA） before and after autoclaving. Methods Turbimetric Assay （TA）, the pH and the diameter of Nanometer-Particles （NPs） were applied to this comparison. Results The pH was slightly decreased during the autoclaving, but there was no significant deviation about turbimetric change or diametric change of the NPs. Conclusion Nanometer-aluminium hydroxide adjuvant can be sterilized by autoclaving during the production of bacterin. It was first discribed the changes of NAHA by autoclaving.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第5期81-84,共4页
China Biotechnology
基金
重庆市科委攻关课题资助项目(2006AC1014)
关键词
纳米氢氧化铝佐剂
高温高压灭菌
理化性质
Nanometeraluminium hydroxide adjuvant Autoclaving Physical and chemical properties
