摘要
目的观察藤梨根乙酸乙酯提取物对培养的人食管癌Eca-109细胞的生长抑制作用,探讨藤梨根对人食管癌细胞的作用机制。方法MTT比色法检测藤梨根乙酸乙酯提取物在不同浓度(1μg/ml,10μg/ml,100μg/ml)及不同时间(2h,48h,72h)对Eca-109细胞生长抑制作用,TUNEL法检测其对癌细胞生长的诱导凋亡效应,免疫组化SP法检测凋亡相关蛋白Bax及Bcl-2的表达。结果①藤梨根乙酸乙酯提取物对人食管癌Eca-109细胞的生长抑制作用随着药物浓度的升高和作用时间的延长而增强,其生长抑制率可达到77.5%。②提取物对瘤细胞有明显的凋亡效应,而在对照组,未见有明显凋亡现象。③提取物对瘤细胞作用24h,48h,72h后分别与对照组比较,用药组Bax蛋白表达明显增强(P<0.01);同时伴有Bcl-2表达的减弱,在72h后最明显,差异有显著意义(P<0.01)。结论藤梨根乙酸乙酯提取物能有效抑制人食管癌Eca-109细胞生长。
Objective To observe the inhibitory effect of extracts from Actinidia arguta by ethyl acetate on human carcinoma of esophagus ceUs（Eca-109）, and explore its mechanism. Methods MTT colonmetric assay was used to detect the growth inhibition effects on Eca-109 cells in different concentrations of extracts from Actinidia arguta by ethyl acetate （1 μg/ml, 10 μg/ml, 100 μg/ ml） and at different times （24 h,48 h,72 h）. TUNEL test was performed to observe the apoptosis induced by the extracts （1 μg/ ml, 10 μg/ml, 100 μg/ml）on Eca-109 cells. The expression of Bax and Bcl-2 in tumor cells was measured by immunohistochemical method（SP technique）. Results ①The inhibitory effect of the extracts on Eca-109 cells increased with the increase of concentration and time, and the highest inhibition rate was 77.5%. ②The extracts could significantly induce apoptosis of Eca-109 cells, but not in control group. ③Compared with control group, the expression of Bax in tumor cells increased after 24 h, 48 h, 72 h（P 〈 0.01 ）, but the expression of Bcl-2 decreased（P 〈 0.01 ）, particularly after 72 h. Conclusion The extracts from Actinidia arguta by ethyl acetate have good inhibitory effect on Eca-109 cells.
出处
《山西医科大学学报》
CAS
2007年第5期413-416,F0003,共5页
Journal of Shanxi Medical University
