摘要
高粱(Sorghum bicolor(L.)Moench)质核互作雄性不育类型有7种,即A1,A2,A3,A4,A5,A6和9E。对于雄性不育机理的研究以往都集中在A1类型上。本文运用SSR方法分析了高粱亲本622A2,晋粱5号,它们的杂交种622A2×晋粱5号,及其F2代323个单株的DNA,从60对SSR引物中筛选到与不育基因连锁的SSR标记Xtxp65和Xtxp30,分别位于目的基因11.5cM和20.0cM处,其特异带型大小分别约为125bp和250bp。分子标记的有效利用有利于优良高粱不育系的选择,也为基于作图的基因分离奠定了基础。
The discovery of cytoplasmic male sterility （CMS） has greatly facilitated the utilization of heterosis in sorghum production. Seven types of sorghum （Sorghum bicolor L. Moench） cytoplasmic-male sterility （CMS） have been identified including A1-A6 and 9E, but only A1 （milo） CMS has been widely studied and used in commercial sorghum hybrid production by far. In this paper we reported the identification of two SSR makers linked to A2 type CMS of sorghum. DNA from 622A2, Jinliang 5, their hybrids and an F2 population comprised of 323 individuals were screened with 60 SSR markers to detect possible linkage with the sterile gene. Xtxp65 was mapped at 11.5 cM and Xtxp30 was mapped at 20.0 cM from the male sterile gene. The sizes of the SSR fragments were about 125 bp and 250 bp, respectively. The availability of molecular markers will facilitate the selection of elite male sterile lines in sorghum breeding and provide the foundation for map-based cloning.
出处
《分子植物育种》
CAS
CSCD
2007年第3期358-362,共5页
Molecular Plant Breeding
基金
山西省自然科学基金项目(2006011085)资助。
关键词
高粱
细胞质雄性不育
SSR
Sorghum, Cytoplasmic-male sterility （CMS）, SSR
