摘要
目的观察糖尿病大鼠早期的骨密度、骨微结构改变及骨组织中BMP-2基因的表达。方法清洁级健康成年雄性Wistar大鼠30只,按照随机数字表将其分为A组(普通饲料对照组)、B组(高脂饲料+链脲佐菌素组)和C组(普通饲料+大剂量链脲佐菌素组)。饲养12周后,分别对各组大鼠进行骨密度、光镜、扫描电镜、骨形态计量学的观察以及BMP-2基因表达等指标的检测。结果与A组相比,B组骨微结构无明显变化(P>0.05),但BMP-2基因表达增高(P<0.05),C组骨微结构明显改变(P<0.05),BMP-2基因表达降低,但差异无统计学意义(P>0.05)。结论不同的糖尿病大鼠模型早期骨微结构及BMP-2基因表达情况改变不完全一致,可能与高胰岛素血症、胰岛素抵抗有一定关系。
Objective To investigate the changes pression in rats at early period of diabetes. Methods of bone density, bone microstructure, bone BMP-2 ex- Thirty SPF 4-month-old male Wistar rats were randomly divided into 3 groups （n = 10 in each group） ： A （fed with common forage as normal control group）, B （fed with high-lipid forage and intraperitoneally injected with STZ to establish type 2 diabetes with insulin resistance）, C （fed with common forage and intraperitoneally injected with STZ to establish type 2 diabetes with insulin secretion defaulting）. The bone histomorphology, bone histomorphometry, bone BMP-2 expression were investigated 12 weeks later. Results As compared with group A, bone microstructure was of no obvious changes and bone BMP-2 expression was higher in group B （ P 〈 0.05 ） , and obvious bone breakage was observed in group C （ P 〈 0.05 ）. Conclusion The changes of bone microstructure and bone BMP-2 expression at the early period of diabetes are not consistent absolutely in individual rat model of diabetes. It maybe relate to hyperinsulinism and insulin resistance.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第11期1053-1056,共4页
Journal of Third Military Medical University
关键词
糖尿病
骨微结构
BMP-2
diabetes mellitus
bone microstructure
BMP-2 expression
