摘要
目的优选山茱萸超临界CO2萃取条件及萃取物GC-MS分析。方法采用正交试验,考察萃取压力、萃取温度、CO2流量和时间四因素对山茱萸超临界CO2萃取物得率的影响并对萃取物成分进行GC-MS分析。结果①CO2萃取的最佳条件为:萃取压力35MPa,萃取温度50℃,CO2流量20L/h,萃取时间2h。GC-MS共分离出72个峰,鉴定了其中32种物质,占总组分的84.27%.主要成分为棕榈酸、9,12-十八碳二烯酸、9-十八碳烯酸及其相关酯类。结论优选了山茱萸超临界CO2萃取条件并对萃取物进行了GC-MS分析。
Objective To optimize the extraction condition for volatile components of Cornus officinalis by orthogonal test and analyze the extract by GC-MS. Methods Four factors were chosen in this experiment, including extractive pressure, extractive temperature, CO2flowing rate and time. The extractive components were analyzed by GC-MS. Results The optimal extraction conditions were： pressure at 35 MPa, heating temperature at 50 ℃ ,being 20 L/h and extracting for 2 h. Seventy-two peaks were isolated and 32 components were identified. The major components identified in the extractions were n-hexadecanoic acid, 9,12-octadecadienoinoc acid and 9-octadecenoic acid, etc.. Conclusion This method established to optimize the extraction condition of volatile components of Comus officinalis and analyze the extract by GC-MS.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第11期1079-1082,共4页
Journal of Third Military Medical University
