摘要
目的研究超顺磁性氧化铁纳米粒子(SPIO)标记成肌细胞方法及其对成肌细胞活性的影响。方法常规方法进行成肌细胞培养,应用不同浓度的SPIO标记成肌细胞,Prussianblue染色检测标记率,JC-1及MTT等方法检测SPIO标记对成肌细胞毒性及细胞活力的影响。结果普鲁士蓝染色证实了每个标记细胞胞质内含有多少不等的蓝染铁颗粒,SPIO标记有效率为100%。低浓度的SPIO对成肌细胞的活性无明显影响,当SPIO浓度达89.6μg/ml时影响成肌细胞的活性。结论SPIO标记成肌细胞简单、高效,低浓度时对细胞的活力无明显影响,可用于磁共振对标记细胞进行活体内外无创动态示踪研究。
Objective To investigate the biological characteristics of superparamagnetic iron oxides（SPIO）- labeled myoblasts in vitro.Methods The cultures of myoblasts from rat were used. Myoblasts were labeled with SPIO agent using liposome transfection agent. Labeling efficiency was measured by light microscopy, For evaluating the toxicity and cell viability of various concentrations of SPIO labeled myoblasts. MTY and JC- 1 was used. Results Prussian blue staining confirmediron uptake and showed 100% labeling efficiency. With the concentration of SPIO increasing （5.6 μg/ml - 44.8 μg/ml）, there was no obvious difference in cell viability of myoblasts（ P 〉 0,05）. When the con- centration of SPIO exceeded 89.6 μg/ml, there was a significant difference （ P 〈 0.01 ）. Conclusions SPIO levels are closely correlated with the biological characteristics of myoblasts, Low density of SPIO has no obvious effect on the toxicity and cell viability of SPIO-labeled myoblasts. Myoblastst labeled with SPIO can be tracked by MRI microscopy in clinical scanners.
出处
《河北医药》
CAS
2007年第2期102-104,共3页
Hebei Medical Journal
基金
全军国际合作基金课题(编号:06H0006)
关键词
成肌细胞
超顺磁性氧化铁
磁标记
细胞活力
myoblastst
superparamagnetic iron oxide
magnetic labeled cells
cell viability
