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大鼠坐骨神经结扎后疼痛受体P2X3在背根神经节内的表达变化 被引量:4

The Expression Changes of Nociceptor P2X3 in Dorsal Root Ganglia aider Sciatic Nerve Ligation
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摘要 目的:研究坐骨神经结扎损伤后疼痛受体P2X3在相应背根神经节(dorsal root ganglia,DRG)内的表达变化情况。方法:选取健康成年SD大鼠35只,建立右侧坐骨神经结扎损伤模型,采用免疫组织化学和图像分析技术检测相应L4-6DRG内P2X3的表达情况。结果:正常大鼠L4-6DRG内有大量P2X3免疫阳性神经元,坐骨神经结扎后3d P2X3表达即下调,3,7,14,21和28d其表达呈进行性下降趋势,各时间点与正常和假手术对照比较差异均有统计学意义(P<0.05)。结论:坐骨神经结扎后P2X3在L4-6DRG内表达明显下调,提示其可能在神经源性疼痛中发挥一定的作用。 Objective: To investigate the expression changes of P2X3 in dorsal root ganglia (DRG) after sciatic nerve ligation. Methods: 35 healthy adult SD rats underwent right sciatic nerve ligation. Immunohistochemistry and image analysis were used to detect the expression of P2X3 in L4-6DRG. Results:There were abundant P2X3-positive primary sensory neurons in normal L4-6DRG. The expression of P2X3 was down-regulated 3 days after sciatic nerve ligation. It also showed that a decreasing tendency appeared during the period Of 3-21 days. The expression did not lcturn to the normal level after 28 days. Conclusion:The obvious down-regulation of P2X3 expression in L4-tDRG after sciatic nerve ligation indicates that it may play an important role in neuropathic pain sensation.
出处 《现代生物医学进展》 CAS 2007年第5期680-681,710,共3页 Progress in Modern Biomedicine
基金 湖南省卫生厅资助项目(B2006-142)
关键词 P2X3 坐骨神经 结扎 背根神经节 神经源性疼痛 Tumor chalone Protein purification Antitumor activity Tumor therapy
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参考文献9

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同被引文献20

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