摘要
目的:建立一种新的荧光法测定痕量锰(Ⅱ)。方法:在pH9.5的氨缓冲介质中,Mn2+与1-(2-吡啶偶氮)-2萘酚(PAN)形成配合物,在无水乙醇介质中,λex/λem=543 nm/558 nm,罗丹明6G(Rh6G)与PAN-Mn2+配合物之间发生能量转移,使Rh6G荧光猝灭,从而建立锰(Ⅱ)的测定新方法。结果:在实验最适条件下,Mn2+浓度在12-600 ng/ml范围内,与荧光猝灭程度呈现良好的线性关系(r=0.9998)。方法的检出限为3.55 ng/ml;相对标准差为0.85%-1.28%(n=11);样品加标回收率为95.0%-107.0%。结论:方法灵敏度高、精密度好、操作简便、费用低,用于水样、人发和茶叶中痕量锰的测定,结果满意。
Objective：To set up a new fluorescence method for the determination of trace manganese（ Ⅱ ）. Methods：In the buffer solution of NH3 · H2O - NH4Cl at pH 9.5, Mn^2＋ and PAN reacted to form complex. In dehydrated alcohol, when the excitation and emission wavelength were found to be 543 nm and 558 nm respectively, the fluorescence intensity of Rh6G was diminished greatly due to the energy transfer from Rh6G to complex PAN -Mn^2＋. Based on this phenomenon, we had established a new method for the determination of trace Mn^2＋. Results：Under the optimum experimented conditions, the linear range of the determination was 12 -600 ng/ml（r =0. 9998）. The detection limit for Mn^2＋ was 3.55 ng/ml and RSD =0. 85% - 1.28% （n = 11 ） with the coverage of 95.0% - 107.0%. Conclusion：The method has the advantage of simplicity, high sensitivity, and had been applied to the determination of trace Mn^2＋ in water, human hair and tea samples to achieve satisfactory results.
出处
《中国卫生检验杂志》
CAS
2007年第5期821-822,953,共3页
Chinese Journal of Health Laboratory Technology
