摘要
目的:建立一种超声波快速提取大米中黄曲霉毒素B1的方法,并对提取方法的回收率和准确度进行评价。方法:采用ELISA方法,研究和评价了超声波提取大米中黄曲霉素B1的提取效率。结果:与普通振荡处理相比,采用超声波处理明显增大了提取效率,缩短了提取时间,超声波处理10 min时达到最大提取率,振荡处理30 min才能达到,而且超声波最大提取浓度比振荡处理提高了30.5%。在针对0.1、1和10μg/kg这3个浓度水平做加标回收试验,回收率达到83.0%-95.2%,比振荡处理提高10%左右。结论:超声频率20 KHz,时间为10 min,为提取黄曲霉毒素B1的最佳条件,而且不同超声频率对结果产生的影响不显著。
Objective：To establish a new extraction method for aflatoxin B1 in rice. Methods：The effects of ultrasonic radiation on extraction of aflatoxin BI from naturally contaminated rice were studied by comparing with shaking by machine better extraction efficient. Results ：Time was also saved for the maximum extraction efficient arrived only after 10 minutes. The recoveries of aflatoxin B1 spiked at 0. 1,1 and 10 μg/kg were in the range of 83.0% -95. 2%. Conclusion：The best method is 20 KHz,time is 10 mins. There is no obvious deviation at different ultrasonic frequency.
出处
《中国卫生检验杂志》
CAS
2007年第5期828-829,926,共3页
Chinese Journal of Health Laboratory Technology
