摘要
目的:测定大骨节病患者和正常对照血清中硒和肿瘤坏死因子-α(TNF-α)、血管内皮生长因子(VEGF)和白介素-1(IL-1β)的水平。从细胞因子角度为研究其发病机制提供实验依据。方法:在西藏拉萨尼木县和墨竹工卡县的大骨节病区随机选取大骨节病患者30例(患者组),病区正常人30例(病区内对照组),在拉萨非大骨节病区选健康志愿者30例(病区外对照组),3组人群年龄和性别没有显著性差异。采取静脉血离心制备血清。采用荧光法测定血清Se,酶联免疫吸附测定法(ELISA)检测血清中细胞因子水平。结果:西藏大骨节病病区患者和正常人血清中Se低于非大骨节病区正常人;患者血清中TNF-α、VEGF和IL-1β的水平高于正常。血清Se与TNF-α、IL-1β水平呈负相关趋势。结论:低硒和血清中细胞因子水平升高在KBD的发病过程中起着某种作用。
AIM： To investigate the levels of selenium （Se） and several cytokines （TNF-α, VEGF and IL-1β） in the serum of patients with Kaschin-Beck disease （KBD）. METHODS： 90 subjects were selected, from KBD areas and non-KBD areas of Lhasa, Tibet. Then they were divided into ：3 groups： the group with KBD （30）, the control in KBD areas （30）, and the control in non-KBD areas （30）. There was no obvious difference in sex and age in the three groups. The content of Se in the serum was detected by atomic fluorescent spectrometer and the level of cytokines （TNF-α, VEGF and IL-1β） in the serum were detected by ELISA. RESULTS： The content of Se in the serum of persons living in the KBD-disease areas were lower than that of persons living in non-KBD disease areas. The levels of TNF-α, VEGF and IL-1β in KBD group were higher than those in normal control group （P〈0.05）. The content of Se had negative correlation with the levels of TNF-α and IL-1β in serum. CONCLUSION： The decrease of Se content and increase of the levels of TNF-α, VEGF and IL-1β may play a role in pathogenesis of KBD.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2007年第6期531-532,共2页
Chinese Journal of Cellular and Molecular Immunology
基金
西藏自治区科技厅重点项目(05-56)
西藏民族学院基金资助项目(05MY11)
