摘要
建立了辣根过氧化物酶(HRP)标记兔抗鸡IgG检测鸡传染性支气管炎(IB)抗体的间接ELISA法,其抗原包被浓度为4mg/L,待检血清与酶结合物的最佳稀释度各为180,酶结合物、抗原抗体的作用时间分别为60min,30min,封闭液的浓度和作用时间分别为1%和60min,OD值大于0.287的判为阳性。用此法检测经IBV灭活苗免疫鸡所产蛋孵出的雏鸡的母源抗体,OD值的P/N值为6.0,15d后降至2.0以下。检测11日龄经IBVH120弱毒苗免疫雏鸡的抗体,其P/N值于免疫后第18d达峰值(3.7)。调查的54群发病鸡,其抗体阳性率为100%,P/N值为4.0~7.
An indirect ELISA was established for detection of rabbit anti chicken antibodies conjugation HRP of chicken infectious bronchitis virus.Its concentration of antigen coating and gelatin blocking fluid was 4 mg/L and 1%.Optimal dilution of serum and enzyme IgG complex was 1∶80, active time of enzyme IgG complex, antibody of antigen and blocking fluid was
出处
《中国兽医科技》
CSCD
1997年第2期3-5,共3页
Chinese Journal of Veterinary Science and Technology