期刊文献+

膀胱移行细胞癌组织中PTEN基因第5、8外显子的突变分析

Study on Mutation of PTEN Gene Exon 5 and Exon 8 in Transitional Cell Carcinoma of the Bladder
下载PDF
导出
摘要 目的:了解膀胱移行细胞癌(TCC)组织中PTEN基因第5外显子(Exon5)和第8外显子(Exon8)的突变情况及其与膀胱癌生物学行为的关系。方法:选择突变率较高的PTEN基因Exon5和Exon8,应用银染PCR-SSCP并结合基因测序方法分析46例TCC患者的PTEN突变情况。结果:PTEN的Exon5扩增产物为379bp,所有肿瘤组织均表现为3条迁移带(2条单链和1条双链),没有发现额外迁移带。Exon8扩增产物为550bp,经酶切为两个片段。所有肿瘤组织均表现为6条迁移带(4条单链和2条双链),没有发现额外迁移带。结论:TCC患者的肿瘤组织中PTEN基因Exon5和Exon8的突变不是一个常见事件,PTEN是否还存在其他灭活方式还需要进一步研究。 Objective: To evaluate the mutation of PTEN exon 5 and exon 8 (high mutation frequence) in transitional cell carcinoma (TCC) of the bladder and the relationship between PTEN gene mutation and the biological behavior of TCC. Methods: PTEN exon 5 and exon 8 were selected to examine 46 TCC patients with PCR-SSCP and gene sequencing. Results:PTEN exon 5 and exon 8 PCR products were 379 bp and 550 bp respectively, and exon 8 PCR products were cut into two small fragments. Three bands in exon 5 and six bands in exon 8 were found respectively in all bladder cancers and normal bladder mucosas without extra bands. The SSCP analysis did not show any mutations or deletions in any of the 46 bladder cancer specimens. Conclusion: PTEN mutation was uncommon in bladder cancers, other inactivation forms of PTEN need to be explored.
出处 《天津医药》 CAS 北大核心 2007年第5期346-347,I0005,共3页 Tianjin Medical Journal
关键词 膀胱肿瘤 移行细胞 基因 肿瘤抑制 突变 bladder neoplasms carcinoma, transitional cell genes, tumor suppressor mutation
  • 相关文献

参考文献7

  • 1张利能,查锡良,俞强,贺建宇.人肝细胞癌中抑癌基因PTEN/MMAC1/TEP1的突变分析[J].实验生物学报,2000,33(3):223-227. 被引量:11
  • 2Velickovic M,Delahunt B,McIver B,et al.Intragenic PTEN/MMAC1 loss of heterozygosity in conventional (clear-cell) renal cell carcinoma is associated with poor patient prognosis[J].Mod Pathol,2002,15(5):479-485.
  • 3Salvesen HB,Stefansson I,Kretzschmar EI,et al.Significance of PTEN alterations in endometrial carcinoma:A population-based study of mutations,promoter methylation and PTEN protein expression[J].Int J Oncol,2004,25(6):1615-1623.
  • 4Ali IU,Schriml LM,Dean M.Mutational spectra of PTEN/MMAC1gene:a tumor suppressor with lipid phosphatase activity[J].J Natl Cancer Inst,1999,91(22):1922-1932.
  • 5Cairns P,Evron E,Okami K,et al.Point mutation and homozygous deletion of PTEN/MMAC1 in primary bladder cancers[J].Oncogene,1998,16:3215-3218.
  • 6Liu J,Babaian DC,Liebert M,et al.Inactivation of MMAC1 in bladder transitional-cell carcinoma cell lines and specimens[J].Mol Carcinog,2000,29:143-150.
  • 7Cheung TH,Lo KW,Yim SF,et al.Epigenetic and genetic alternation of PTEN in cervical neoplasm[J].Gynecol Oncol,2004,93(3):621-627.

二级参考文献5

  • 1Wang S I,Clin Cancer Res,1998年,4卷,811页
  • 2Piao Z,Int J Cancer,1998年,75卷,29页
  • 3Li M,生物化学与生物物理学报,1998年,1391卷,256页
  • 4Wang S I,Cancer Res,1997年,57卷,4183页
  • 5Li J,Science,1997年,275卷,1943页

共引文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部