摘要
目的:研究亚砷酸在类风湿关节炎(RA)大鼠中的治疗作用。方法:40只大鼠随机分成4组:正常对照组(C组)、模型组(M组)、低剂量亚砷酸组(LSA1.5mg.kg-1.d-1)、高剂量亚砷酸组(HSA3.0mg.kg-1.d-1)。原位杂交检测各组核因子-κB(NF-κB)、细胞核因子-κB受体活化因子配体(RANKL)mRNA的表达。结果:与C组比较,M组NF-κB、RANKL mR-NA大量表达(P<0.01);与M组比较,LSA组及HSA组NF-κB、RANKL mRNA表达降低(P<0.01),且HSA组更明显。结论:RANKL、NF-κB在类风湿关节炎发生发展中具有重要作用,亚砷酸对类风湿关节炎有治疗作用。
OBJECTIVE To study therapeutic effect of sodium arsenite in the treatment of rhumtoid arthritis rats. METHODS 40 Wistar female rats were randomly divided into 4 groups： normal control group（C）, model group （M）, low concentration sodium arsenite group（LSA） and high concentration sodium arsenite group（HSA）. LSA group and HAS group were treated with sodium arsenite （1.5 mg·kg^-1·d^-1 and 3 mg·kg^-1·d^-1） through abdominal injection,the C group and the M group were treated with saline（2 mL/d）. The expressions of NF-κB and RANKL mRNA of synovium were measured by in situ hybridization. RESULTS Compared with C group,the expressions of NF-κB and RANKL mRNA in the synovium were up-regulated in the M group（P〈0. 01） and were inhibited by sodium arsenite treatment （P〈0. 01 ）, especially in the HAS group. CONCLUSION NF-κB and RANKL may play important roles in the development of RA. Sodium arsenite can down regulate the expressions of NF-κB and RANKL mRNA and may have some therapeutic effects in rheumatoid arthritid.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2007年第5期593-595,共3页
Chinese Journal of Hospital Pharmacy
基金
贵州省省长基金(编号:2004.12)
