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基质辅助激光解析电离质谱靶上样品前处理方法研究进展 被引量：3

Pre-treatment Methods On-probe for MALDI Mass Spectrometry
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摘要 基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)技术已成为目前蛋白质组学研究中的经典技术。在该技术的成功应用过程中,合适的样品前处理方法起着首要和关键的作用。只有将合适的样品前处理方法与合适的有机基质结合起来,才能成功实现对多肽和蛋白质等生物大分子的准确鉴定。随着质谱分析向高灵敏度、高准确度、高通量的方向发展,出现了许多新的,可直接在MALDI靶上前处理的方法,极大地简化了对生物样本的处理步骤,同时减少了样品的损失。特别是将一些纳米材料用于MALDI靶上进行样品的前处理,为样品前处理方法的研究开辟了广阔的应用前景。 Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS） became a classical tool for the proteome analysis. In the analytical process, proper pre-treatment method of samples, the same as the selection of matrix played a major role for the successful identifications of proteins and peptides. As sensitivity, mass accuracy and throughput of MS analysis become important parameters, lots of pretreatment methods on-probe, including those promising protocols based on nano-particles materials, which have been used to improve the analysis of biological samples.
作者 卫军营 蔡耘 钱小红 张养军
机构地区 军事医学科学院放射与辐射医学研究所北京蛋白质组研究中心
出处 《质谱学报》 EI CAS CSCD 2007年第2期122-128,共7页 Journal of Chinese Mass Spectrometry Society
基金 国家重点基础研究规划项目(2004CB520802) 国家自然科学基金重点项目(20635010) 国家自然科学基金(30321003 20405017 20505018 20505019) 北京市科委重大项目(H030230280190)
关键词 基质辅助激光解析电离质谱 靶上前处理方法 matrix-assisted laser desorption/ionization mass spectrometry pretreatmentmethods on-probe
分类号 O657.63 [理学—分析化学]
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参考文献45

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共引文献24

同被引文献29

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引证文献3

二级引证文献8

质谱学报
2007年 第2期

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