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焦磷酸测序技术及应用 被引量：11

Pyrosequencing and Its Application
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摘要 焦磷酸测序(pyrosequencing)技术是一种新的DNA序列分析技术,其特点是操作简便、大通量、自动化,适合大量样本的快速检测,无须进行电泳、DNA序列无须荧光标记等,是一个理想的遗传分析技术平台。文章对焦磷酸测序技术原理及近年来在单核苷酸多态性研究、微生物鉴定分型和DNA甲基化等方面的应用作一简要综述。 Pyrosequencing is a DNA sequencing technique based on the detection of released pyrophosphate （PPi） during DNA synthesis. Pyrosequencing is characterized by easy handling, high throughput and automation. This article discusses the principles and the operating procedures of this new technology and the latest advances in its application in single nucleotide polymorphism （SNP） genotyping, microbial typing and CpG methylation analysis.
作者 汤华阳 杜卫东 张学军
机构地区 安徽医科大学皮肤病研究所安徽医科大学第一附属医院皮肤科教育部重要遗传病基因资源利用重点实验室
出处 《医学分子生物学杂志》 CAS CSCD 2007年第3期272-275,共4页 Journal of Medical Molecular Biology
关键词 焦磷酸测序 单核苷酸多态性 微生物鉴定 甲基化 pyrosequencing single nucleotide polymorphism genotyping microbial typing CpG methylation analysis
分类号 Q819 [生物学—生物工程]
  • 相关文献

参考文献20

  • 1[1]GHARIZADEH B,NORDSTROM T,AHMADIAN A,et al.Long-read pyrosequencing using pure 2'-deoxyadenosine-5'-0'-(1-thiotriphosphate) Sp-isomer[J].Anal Biechem,2002,301(1):82-90.
  • 2程绍辉,梁明华,李泽琳,张霆,张珑,马洪涛,刘哲伟,曾毅.焦磷酸测序技术在确认北京严重急性呼吸综合征(SARS)病毒株并检测基因突变中的应用[J].病毒学报,2005,21(3):168-172. 被引量：5
  • 3[3]INNINGS A,KRABBE M,ULLBERG M,et al.Identification of 43 streptoeoecus species by pyrosoquencing analysis of the rnpB gene[J].J Clin Microbio,2005,43(12):5983-5991.
  • 4[4]JUREEN P,ENGSTRAND L,ERIKSSON S,et al.Rapid detection of rifampin resistance in mycobacterium tuberculosis by pyrosequencing technology[J].J Clin Microbiol,2006,44(6):1925-1929.
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  • 9杜卫东,吕永梅,汤华阳,黄叔仁,陈大本,何祥成,王永清,孙松,张学军,徐昌娟（校）.Leber遗传性视神经病变线粒体DNA原发性突变位点的分析[J].疾病控制杂志,2006,10(6):574-579. 被引量：9
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二级参考文献20

  • 1Ching A, Rafalski A. Rapid genetic mapping of tests using SNP pyrosequencing[J ]. Cell Mol Biol Lett,2002,7:803- 810.
  • 2Agaton C, Unnerberg P, Sievertzon M, et al. Gene expression analysis by signature pyrosequencing[ J ]. Gene, 2002,289: 31 - 39.
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  • 6The Chinese SARS Molecular Epidemiology Consortium · Molecular evolution of the SARS-coronavirus during the course of the SARS epidemic in China[J]. Science,2004,303(5664): 1666 - 1669.
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  • 8Ruan Y J, Lin C,Ai Eb W L,et al. Comparative full-lengh genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection [ J ]. The Lancet,2003,361(24): 1779 - 1784.
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  • 10Pietropaolo M, Becker DJ, Laporte RE, et al. Progression to insulinrequiring diabetes in seronegative prediabetic subjects :the role of two HLA-DQ high-risk haplotypes. Diabetological,2002,45:66-73.

共引文献13

同被引文献144

引证文献11

二级引证文献19

医学分子生物学杂志
2007年 第3期

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