摘要
为了改善羟基磷灰石(HAP)的脆性和新骨诱导性,采用共沉淀法合成HAP,盐溶法制备丝素蛋白(SF),在胶体状态下将HAP和SF复合得到了SF/HAP复合材料。采用扫描电镜(SEM)、X射线衍射(XRD),傅立叶红外光谱(FIR)对复合材料结构和化学组成进行了分析,在模拟体液中检验了复合材料的生物活性,并对其抗压强度进行了测定。结果表明:HAP与SF在纳米尺度进行了复合,复合材料中SF主要以β-折叠构象存在,酰胺Ⅴ红外特征峰消失,β-折叠构象的其他峰发生了移动,表明HAP与SF间存在化学结合;模拟体液中浸泡18天后,复合材料表面形成了片层状的HAP;与纯的HAP晶体比较,复合材料结构稳定,具有较好生物活性和骨诱导性,其抗压强度可达63 MPa,可望成为理想的骨组织替换和工程支架材料。
In order to improve hydroxyapatite（HAP） brittleness and osteo-inductivity, HAP was prepared using the co-precipitation method. Silk-fibroin （SF） was obtained by salt-resolving. The SF/HAP composite was derived by mixing HAP sols with SF sols. The phase composition and morphology of HAP and SF/HAP composites were investigated using X-ray diffraction （XRD）, scanning electron microscopy （SEM） and Fourier infrared spectroscopy （FIR）. The compressive strength of the SF/HAP composites was also measured. The biological activity of the SF/ HAP composites was observed while thermostatic held in simulated body fluid （SBF）. The results show that HAP mixes with SF in the SF/HAP composites at the nano-scale. The SF in the SF/HAP composites mainly has the typical silk Ⅱ conformation （β-sheet）. The pectral peak of acyl V of β-sheet conformation disappears and the positions of the other spectral peaks of β-sheet conformation in the SF/HAP composites are changed, which indicates that the chemical bonds are formed between SF and HAP granules. When the SF/HAP composite is dipped in SBF for eighteen days, the needle-llke HAP sheet is formed on the surface. The compressive strength of the SF/HAP composites reaches 63 MPa. It is clear that the SF/HAP composite is a desirable biomedical material for the repairing of bone defects with an excellent bioactivity, osteo-inductivity and high mechanical strength.
出处
《复合材料学报》
EI
CAS
CSCD
北大核心
2007年第3期141-146,共6页
Acta Materiae Compositae Sinica
基金
西安市科技局重点技术攻关项目(GG200354)
陕西省2004年自然科学基金资助(2004B24)
陕西科技大学B类科技创新团队经费资助(sust-B104)
关键词
羟基磷灰石
复合材料
丝素蛋白
骨诱导性
抗压强度
hydroxyapatite （HAP）
silk-fibroin （SF）
SF/HAP composites
osteo-inductivity
compressive strength
