摘要
目的探讨HIV Tat蛋白对CD4^＋ T淋巴细胞bcl-2表达的影响，并探讨肿瘤坏死因子相关凋亡诱导配体（TRAIL）与经HIV Tat蛋白刺激后的CD4^＋ T淋巴细胞凋亡的相互关系。方法用Western blot方法测定经HIV Tat蛋白刺激后，CD4＋ T淋巴细胞表达bcl-2的水平，以λ-氨基放线菌素D（7-AAD）和AnnexinV染色方法测定CD4＋ T淋巴细胞的凋亡。结果HIV Tat蛋白能明显增加CD4＋ T淋巴细胞bcl-2的表达，以100ng/ml为最佳浓度；7-AAD染色结果表明100ng/ml重组TRAIL能诱导53．85％±2．63％的CD4^＋ T淋巴细胞发生凋亡，如CD4^＋ T淋巴细胞经HIV Tat蛋白刺激后，则仅有16．04％±5．26％的细胞发生凋亡，此作用能被多克隆抗-Tat蛋白抗体抑制。Annexin V染色取得了同样的结果。结论经HIV Tat蛋白刺激后CD4^＋ T淋巴细胞bcl-2的表达明显增加，并能抑制由重组TRAIL诱导的细胞凋亡，提示HIV Tat蛋白是导致HIV在CD4^＋ T淋巴细胞内持续感染的重要调节蛋白，在HIV感染中起十分重要作用。
Objective To investigate the effect of HIV Tat protein on bcl-2 expression in CD4^＋ T lymphocytes, and Tat-stimulated CD4^＋ T lymphocytes apoptosis induced by TNF-a related apoptosis induced ligand （TRAIL）. Methods Western blot was used to detect the bcl-2 expression in CD4^＋ T lymphocytes stimulated by HIV Tat protein, and 7-AAD and Annexin V were used to detect apoptosis of Tat-stimulated CD4^ T lymphocytes induced by TRAIL. Resdts HIV Tat protein could increase bcl-2 expression in CD4^＋ T lymphocytes.7-AAD staining result showed that 53.85%±2.63% CD4^ T lymphoeytes had apoptosis after being treated with 100 ng/ml recombinant TRAIL.If CD4^ T lymphocytes were pre-stimulated with HIV Tat,only 16.04% ±5.26% cells showed apoptosis.This effect can be inhibited by polyckme anti-Tat. Annexin V staining showed the same results. conclusion HIV Tat protein increases bcl-2 expression in CD4^＋ T lymphocytes,which inhibits apoptosis induced by TRAIL. HIV Tat protein may play an important role in mechanisms of HIV persistent infection in CD4^＋ T lymphocytes.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2007年第4期302-305,共4页
Chinese Journal of Microbiology and Immunology
基金
国家教育部留学回国人员科研启动基金
