摘要
目的:研究鹿藿醋酸乙酯提取物(Rhynchosia volubilis Lour ethyl acetate extract,RVLEAE)的抗生育作用及化学成分分析。方法:①用溶剂萃取法提取有效部位,减压蒸馏浓缩制成浸膏;②抗生育实验:80只雄性小鼠分为4组即生理盐水(NS)对照组,阳性药物(雷公藤多苷10 mg·kg^(-1)·d^(-1))对照组,鹿藿醋酸乙醇提取物低、高剂量(醋酸乙酯浸膏100,400 mg·kg^(-1)·d^(-1))组,灌胃,每天1次,连续给药11周。给药2,10周后,合笼交配,1周后处死部分雄鼠观察附睾精子、附睾及睾丸病理变化、血清睾酮含量以及体重变化情况;分笼10 d后处死雌鼠,观察妊娠率、活胎数、死胎数;③化学成分分析用柱层析分离、薄层色谱初步分析及红外色谱、核磁共振波谱定性分析。结果:RVLEAE连续用药2周,小鼠妊娠率明显下降。妊娠抑制率明显增高。连续用药10周后作用仍较明显。3周时的精子数量,在高、低剂量组减少量为明显。连续给药后精子数量减少。RVLEAE对小鼠睾丸及附睾组织影响不大。血清睾酮含量,阳性对照组明显增高,而高、低剂量组与生理盐水对照组差异无显著性。RVLEAE的主要成分为糖及苷类、醇和酚类化合物。3号组分被鉴定为β-甲基2,4-二甲氧基-L-吡喃岩藻糖苷。6号组分被鉴定为α,β-D-吡喃葡萄糖和α,β-甲氯基-D-吡喃葡萄糖单糖的混合物。结论:RVLEAE有抗生育作用,作用部位主要在附睾,作用机制可能是糖苷类化合物干扰了精子在附睾的成熟。
Objective： To study the effect of Rhynchosia volubilis Lour ethyl acetate extract （RVLEAE） on male mouse procreation and analyse their chemical composition. Method： With the method of solvent extraction, RVLEAE was extracted and concentrated. In the experiment of mice, 80 male mice were randomly and equally divided into four groups： Normal Saline control, positive control with 0. 1% triperygium wilfordii glycoside, 100 mg ·kg^-1·d^-1 RVLEAE and 400 mg ·kg^-1·d^-1 RVLEAE. Every mouse was fed with drug 0. 01 mL · g^-1, once a day, ig, for eleven consecutive weeks. After two and 10 weeks, male and female mouse naturally mated for one week. The pregnancy rate, number of fetus and nonviable fetus, the viability of spermatozoon in the epididymis cauda, pathological change of testis and epididymis were observed in this experiment. In the analysis of chemical composition, RVLEAE were separated with column chromatography, and chemical compositions were identified with thinlayer chromatography, infrared chromatography and nuclear magnetic resonance. Result. The pregnancy rate of mice was markedly decreased. The number and viability of spermatozoon were slightly reduced in Ⅰ and Ⅱ after two and 10 weeks, but the pathological changes of testis and epididymis were markedly occurred. Main chemical compositions were identified as saccharide, glycosides especially analog of fucose, alcohols, and phenols. Conclusion： RVLEAE can inhibit the procreation of male mice, and inhibitory target tissue may be the epididymis. Active mechanism of RVLEAE may be that glycosides interfere the maturation of spermatozoon in the epididymis cauda.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第11期1072-1076,共5页
China Journal of Chinese Materia Medica
基金
湖北省计划生育委员会资助项目(Js200301)
关键词
鹿藿醋酸乙醇提取物
抗生育作用
附睾
岩藻糖苷
Rhynchosia volubilis Lour ethyl acetate extract
inhibit the procreation
epididymis
analog of fucose
