摘要
目的通过观察健康供者外周血CD34+细胞黏附分子表达的变化,探讨粒细胞集落刺激因子(G-CSF)动员的机制及其对供者的影响。方法应用流式细胞仪分析15名健康供者在接受G-CSF10μg.kg-1.d-1动员前(Pre-G)、动员d4和停止动员后7d(Pro-G)外周血CD34+细胞比例及其表面黏附分子非常延迟抗原-5(VLA-5,CD49e)和L-选择素(CD62L)的表达情况。结果G-CSF动员后供者外周血CD34+细胞比例较动员前增高5-10倍,停止动员后恢复至动员前水平。G-CSF动员后CD34+CD49e+细胞比例(97.74%)明显高于动员前(79.95%),停止动员后7d CD34+CD49e+细胞比例基本恢复至动员前水平;CD34+CD62L+细胞比例在G-CSF动员过程中无明显改变;CD34+细胞表面CD49e与CD62L的平均荧光强度于动员后呈减弱趋势,但无显著统计学意义。结论G-CSF动员后d4可显著增加供者外周血CD34+细胞比例,可致CD34+CD49e+细胞比例一过性增加,但不影响CD34+CD62L+细胞群的比例。
Objective To study the changes of the expression of adhesion molecules on peripheral blood CD34^＋ cells during recombinant human granulocyte colony stimulating factor （rhG-CSF） mobilization, and to study the influence of rhCrCSF on donors. Methods Fifteen healthy blood donors were subcutaneously injected with rhG-CSF（10μg · kg^-1 · d^-1 ）for 4 to 6 days. The expressions of very late antigen 5（VLA-5, CD49e） and L-selectin （CD62L） on CD34^＋ cells were examined by flow cytometry before mobilization, the fourth day during mobilization and the seventh day after mobilization. Results The percentage of CD34^＋ cells, especially CD34^＋ CD49e^＋ cells, increased significantly during mobilization, reaching the peak on the forth day, but declined to normal level when the mobilization stopped. The percentage of CD34^＋ CD62L^＋ cells didn＇t show any significant change during the mobilization. Conclusion rhG-CSF could increase the percentage of CD34^＋ CD49e^＋ cells in peripheral blood, but the percentage was reduced one week after the mobilization, rhG-CSF doesn＇t affect the percentage of CD34^＋ CD62L^＋ cells.
出处
《中国输血杂志》
CAS
CSCD
2007年第2期105-107,共3页
Chinese Journal of Blood Transfusion
